Automated cell processing instruments comprising reagent cartridges

• US 10,376,889 B1
• Filed: 04/10/2019
• Issued: 08/13/2019
• Est. Priority Date: 04/13/2018
• Status: Active Grant

• Current Assignee: Inscripta, Inc.
• Original Assignee: Inscripta, Inc.
• Inventors: Masquelier, Don, Belgrader, Phillip, Van Hatten, Brian, Bernate, Jorge, Chabansky, Bruce
• Primary Examiner(s): Hobbs, Michael L
• Application Number:
  US16/380,828
• Time in Patent Office: 125 Days
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/04 Closures and closing means
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 3/527 for a plurality of reagents
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: B01L 7/02 Water baths; Sand baths; Air baths
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 23/44 Multiple separable units; Modules
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12M 41/18 Heat exchange systems, e.g. heat jackets or outer envelopes
• CPC Class Codes: C12M 41/48 Automatic or computerized control automatic analysis G01N35/00
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.

Claims

1. A reagent cartridge comprising:

a thermal block with block reservoirs defined therein;

a cover disposed upon the thermal block with cover reservoirs defined therein, wherein the cover reservoirs and block reservoirs are coincident and together define reservoirs in the reagent cartridge;

an insert comprising a flow-through electroporation device;

a script readable by a processor; and

at least two additional inserts configured to reside within the reservoirs in the reagent cartridge.

2. The reagent cartridge of claim 1, wherein one or more of the inserts comprises tubes.

3. The reagent cartridge of claim 1, wherein one or more of the inserts comprises a reservoir coincident with the reservoirs in the reagent cartridge.

4. The reagent cartridge of claim 1, wherein the flow-through electroporation device comprises:

an inlet and inlet channel for introduction of a cell sample to the flow-through electroporation device;

an outlet and outlet channel for exit of the cell sample from the flow-through electroporation device;

a flow channel intersecting and positioned between the inlet channel and outlet channel with a constriction in the flow channel positioned between the inlet channel and outlet channel; and

two or more electrodes, wherein at least one of the electrodes is positioned in the flow channel between the intersection of the flow channel with the first inlet channel and the constriction in the flow channel and at least one of the electrodes is positioned between the constriction in the flow channel and the intersection of the flow channel with the outlet channel, and wherein the two or more electrodes are in fluid communication with the cell sample in the flow channel but are not in the flow path of the cell sample in the flow channel nor do the two or more electrodes define the constriction of the flow channel, and wherein the two or more electrodes are configured to apply an electric pulse or electric pulses to a cell sample.

5. The reagent cartridge of claim 1, wherein one, two or all three of the cover, thermal block, and at least one of the at least two inserts are fabricated from stainless steel, aluminum, polyvinyl chloride, cyclic olefin copolymer (COC), polyethylene, polyamide, polyethylene, polypropylene, acrylonitrile butadiene, polycarbonate, polyetheretheketone (PEEK), poly(methyl methylacrylate) (PMMA), polysulfone, or polyurethane.

6. The reagent cartridge of claim 5, wherein one, two or all three of the cover, thermal block, and at least one of the at least two inserts are fabricated from cyclic olefin copolymer (COC).

7. The reagent cartridge of claim 1, wherein the reagent cartridge is configured to accommodate four or more inserts.

8. The reagent cartridge of claim 1, wherein the reagent cartridge can be cooled to 0°

C. and heated to 40°

C.

9. The reagent cartridge of claim 1, wherein the script comprises information on the contents of the inserts.

10. An automated multi-module cell processing instrument comprising the reagent cartridge of claim 1, wherein the automated multi-module cell processing instrument comprises a processor, and the script readable by a processor comprises commands for performing three or more processes in the automated multi-module cell processing instrument.

11. The automated multi-module cell processing instrument of claim 10, wherein the script readable by a processor comprises commands for performing all processes in the automated multi-module cell processing instrument.

12. The automated multi-module cell processing instrument of claim 11, further comprising a cell growth module.

13. The automated multi-module cell processing instrument of claim 12, wherein the cell growth module comprises a rotating growth vial.

14. The automated multi-module cell processing instrument of claim 11, further comprising a cell concentration module.

15. The automated multi-module cell processing instrument of claim 14, wherein the cell concentration module comprises a tangential flow filtration device.

16. The automated multi-module cell processing instrument of claim 11, further comprising a storage module.

17. The automated multi-module cell processing instrument of claim 11, further comprising a nucleic acid assembly module.

18. The automated multi-module cell processing instrument of claim 11, further comprising an editing module.

19. The automated multi-module cell processing instrument of claim 11, further comprising a singulation module.