System and method for temperature referencing for melt curve data collection
First Claim
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1. A system for performing melt curve data collection comprising:
- a container comprising at least three chambers, wherein at least two of the chambers are spatially separated from each other, and contain a temperature reference material, and at least one chamber contains a DNA test sample;
a heating system to heat the container,a detection system, wherein a detectable signal from the temperature reference material provides feedback to the heating system; and
a control system for determining a spatial temperature gradient due to temporal fluctuation within the container to calculate a temperature at a location of the DNA test sample based on temperature results from the temperature reference material that surrounds the location of the DNA test sample.
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Abstract
The present invention relates to systems and methods of temperature referencing for melt curve data collection. More specifically, the present invention relates to systems and methods for collecting DNA melt curve data for a DNA sample and a temperature reference material.
60 Citations
24 Claims
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1. A system for performing melt curve data collection comprising:
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a container comprising at least three chambers, wherein at least two of the chambers are spatially separated from each other, and contain a temperature reference material, and at least one chamber contains a DNA test sample; a heating system to heat the container, a detection system, wherein a detectable signal from the temperature reference material provides feedback to the heating system; and a control system for determining a spatial temperature gradient due to temporal fluctuation within the container to calculate a temperature at a location of the DNA test sample based on temperature results from the temperature reference material that surrounds the location of the DNA test sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 21, 22)
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11. A system for performing melt curve data collection, said system comprising:
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a container comprising at least three chambers that are refillable without moving the container, wherein at least two of the chambers are spatially separated from each other and contain a temperature reference material, and at least one chamber contains a DNA test sample; a heating system to heat the container; a detection system, wherein a detectable signal from the temperature reference material provides feedback to the heating system; a control system for determining a spatial temperature gradient due to temporal fluctuation within the container to calculate a temperature at a location of the DNA test sample based on temperature results from the temperature reference material that surrounds the location of the DNA test sample. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 23, 24)
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Specification