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System and method for temperature referencing for melt curve data collection

  • US 10,376,891 B2
  • Filed: 07/18/2016
  • Issued: 08/13/2019
  • Est. Priority Date: 06/23/2008
  • Status: Active Grant
First Claim
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1. A system for performing melt curve data collection comprising:

  • a container comprising at least three chambers, wherein at least two of the chambers are spatially separated from each other, and contain a temperature reference material, and at least one chamber contains a DNA test sample;

    a heating system to heat the container,a detection system, wherein a detectable signal from the temperature reference material provides feedback to the heating system; and

    a control system for determining a spatial temperature gradient due to temporal fluctuation within the container to calculate a temperature at a location of the DNA test sample based on temperature results from the temperature reference material that surrounds the location of the DNA test sample.

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