×

Mice that produce hybrid antibodies

  • US 10,378,038 B2
  • Filed: 09/25/2013
  • Issued: 08/13/2019
  • Est. Priority Date: 02/16/2001
  • Status: Expired due to Fees
First Claim
Patent Images

1. A transgenic mouse, whose genome comprises:

  • (a) unrearranged human immunoglobulin heavy chain V, D, and J gene segments contained on a genomic DNA fragment larger than 20 kb, wherein the unrearranged human heavy chain V, D, and J gene segments in situ replace mouse endogenous immunoglobulin heavy chain V, D, and J gene segments, and the unrearranged human immunoglobulin heavy chain V, D, and J gene segments are operably linked to an endogenous mouse heavy chain constant region gene, wherein the mouse heavy chain constant region gene is located at an endogenous mouse immunoglobulin heavy chain constant region locus, wherein the unrearranged human immunoglobulin heavy chain V, D, and J gene segments are present in the germline of the mouse, wherein rearrangement of the human immunoglobulin heavy chain V, D, and J gene segments in the mouse results in a rearranged human heavy chain variable region gene linked to the mouse heavy chain constant region gene; and

    (b) unrearranged human immunoglobulin kappa chain V and J gene segments, wherein the unrearranged human kappa chain V and J gene segments in situ replace mouse endogenous immunoglobulin kappa chain V and J gene segments, and the unrearranged human immunoglobulin kappa chain V and J gene segments are operably linked to an endogenous mouse kappa chain constant region gene, wherein the mouse kappa chain constant region gene is located at an endogenous mouse immunoglobulin kappa chain constant region locus, wherein the unrearranged human immunoglobulin kappa chain V and J gene segments are present in the germline of the mouse, wherein rearrangement of the human immunoglobulin kappa chain V and J gene segments in the mouse results in a rearranged human kappa chain variable region gene linked to the mouse kappa chain constant region gene;

    wherein the mouse in response to an antigen produces a hybrid antibody that comprises a human heavy chain variable region encoded by the rearranged human heavy chain variable region gene, a mouse heavy chain constant region encoded by the mouse heavy chain constant region gene, a human kappa chain variable region encoded by the rearranged kappa chain variable region gene, and a mouse kappa chain constant region encoded by the mouse kappa chain constant region gene, and wherein the mouse does not produce fully human antibodies.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×