Fiber sampler for recovery of bioaerosols and particles

• US 10,378,042 B2
• Filed: 02/19/2013
• Issued: 08/13/2019
• Est. Priority Date: 04/08/2004
• Status: Active Grant

• Current Assignee: Aerosol Dynamics, Inc., Research Triangle Institute
• Original Assignee: Research Triangle Institute
• Inventors: Ensor, David S., Walls, Howard Jerome, Foarde, Karin K., Hering, Susanne Vera, Spielman, Steven Russel
• Primary Examiner(s): Woodward, Nathaniel T
• Application Number:
  US14/378,768
• Publication Number:
  US 20150024379A1
• Time in Patent Office: 2,366 Days
• Field of Search: 73 2801
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Abstract

An aerosol collection system and method. The system includes a bio-aerosol delivery device configured to supply bioparticles in a gas stream, a moisture exchange device including a partition member coupled to the gas stream and configured to humidify or dehumidify the bioparticles in the gas stream, and an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles. The method includes delivering bioparticles in a gas stream, humidifying or dehumidifying the bioparticles in the gas stream by transport of water across a partition member and into a vapor phase of the gas stream, and collecting the bioparticles by a collection medium.

Claims

1. A method for collecting aerosols, comprising:

providing an aerosol humidifying device having therein a) a first temperature and humidity controllable gaseous environment and b) a second temperature and humidity controllable gaseous environment, the first and second temperature and humidity controllable gaseous environments separated only by a permeable partition member having a polymeric wall;

delivering, as the aerosols to be collected, bioparticles from outside into a first gas stream of the first temperature and humidity controllable gaseous environment;

humidifying or dehumidifying the bioparticles in the first gas stream of the first temperature and humidity controllable gaseous environment by transport of water from a second gas stream of the second temperature and humidity controllable gaseous environment across the permeable partition member and into a vapor phase of the first gas stream, the permeable partition member preventing contamination between a) the first gas stream of the first temperature and humidity controllable gaseous environment including the bioparticles and b) the second gas stream of the second temperature and humidity controllable gaseous environment; and

collecting the bioparticles by a collection medium.

2. The method of claim 1, wherein humidifying or dehumidifying the particles comprises maintaining a relative humidity greater than 5%.

3. The method of claim 1, wherein

said polymeric wall comprising at least one of a permeable material, a semi-permeable membrane material, or a polymeric ionomer, and

the polymeric wall surrounding surrounds the first gas stream including the bioparticles.

4. The method of claim 1, wherein humidifying or dehumidifying the particles comprises transporting the water through the said polymeric wall comprising at least one of:

a copolymer of perfluoro-3,6-dioxa-4-methyl-7octene-sulfonic acid and tetrafluoroethylene;

polyethylene oxide, polyvinyl alcohol, cellulose ether and starch) and copolymers thereof; and

polyacrylic acid, polyacrylamide, polyisopropyl acrylamide, polystyrene sulfonic acid, polyvinyl pyridine and polyamino acid and copolymers and salts thereof.

5. The method of claim 1, further comprising:

stabilizing at least one of a temperature and relative humidity of the aerosol collection medium prior to collecting the bioparticles.

6. The method of claim 1, further comprising:

controlling a temperature and a relative humidity along a gas-flow axis of the membrane.

7. The method of claim 6, further comprising:

adjusting the temperature and the relative humidity such that the particles in the first gas stream transition from outside ambient conditions to a target temperature and relative humidity condition.

8. The method of claim 7, further comprising:

adjusting the temperature and the relative humidity to one of the following sets of conditions;

2°

C. to 10°

C. and RH of 5 to 95%

2°

C. to 10°

C. and RH of 70 to 85%;

1°

C. to 8°

C. and RH of 5 to 95%;

1°

C. to 8°

C. and RH of 70 to 85%;

2°

C. to 6°

C. and RH of 5 to 95%;

2°

C. to 6°

C. and RH of 70 to 85%;

4°

C. and RH of 5 to 95%;

or

4°

C. and RH of 70 to 85%.

9. The method of claim 7, further comprising:

adjusting a temperature and a relative humidity of the bioparticle while transitioning from outside ambient conditions to the target temperature and relative humidity condition along a controlled path of temperature and humidity,

said controlled path comprises a two-stage system including a first stage having a relative humidity in a range from 40-60% at ambient temperature and a second stage below ambient temperature while maintaining the relative humidity in said range from 40-60%.

10. The method of claim 1, further comprising:

providing a viability enhancing material to the collection medium.

11. The method of claim 1, wherein providing a viability enhancing material comprises providing at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth to the collection medium.

12. The method of claim 1, wherein collecting the bioparticles comprises collecting at least one of pox viruses, filoviruses, arenaviruses, alphavirus, brucella species, burkholderia mallei, yersinia pestis and coxiella burnetii on the collection medium.

13. The method of claim 1, wherein

said polymeric wall comprises at least one tubular wall separating the first temperature and humidity controllable gaseous environment from the second temperature and humidity controllable gaseous environment.

14. The method of claim 13, wherein said polymeric wall comprises plural tubular walls separating the first temperature and humidity controllable gaseous environment from the second temperature and humidity controllable gaseous environment.

15. The method of claim 1, wherein said polymeric wall comprises a flat sheet membrane separating the first temperature and humidity controllable gaseous environment from the second temperature and humidity controllable gaseous environment.