Apparatus and methods for parallel processing of microvolume liquid reactions
First Claim
1. A method for conducting a polynucleotide reaction, the method comprising:
- introducing (1) a liquid sample comprising a nucleic acid, (2) reagents, and (3) a hydrophobic fluid into a cavity of a substrate having a first surface and a second surface and the cavity extending through the substrate from the first surface to the second surface;
coating the first surface of the substrate with a hydrophobic fluid; and
conducting a polynucleotide reaction on the liquid in the cavity.
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Abstract
Disclosed herein are apparatuses and methods for conducting multiple simultaneous micro-volume chemical and biochemical reactions in an array format. In one embodiment, the format comprises an array of microholes in a substrate. Besides serving as an ordered array of sample chambers allowing the performance of multiple parallel reactions, the arrays can be used for reagent storage and transfer, library display, reagent synthesis, assembly of multiple identical reactions, dilution and desalting. Use of the arrays facilitates optical analysis of reactions, and allows optical analysis to be conducted in real time. Included within the invention are kits comprising a microhole apparatus and a reaction component of the method(s) to be carried out in the apparatus.
289 Citations
18 Claims
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1. A method for conducting a polynucleotide reaction, the method comprising:
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introducing (1) a liquid sample comprising a nucleic acid, (2) reagents, and (3) a hydrophobic fluid into a cavity of a substrate having a first surface and a second surface and the cavity extending through the substrate from the first surface to the second surface; coating the first surface of the substrate with a hydrophobic fluid; and conducting a polynucleotide reaction on the liquid in the cavity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification