Method and compositions for improving selective catabolysis in cells of keratin surfaces

• US 10,383,815 B2
• Filed: 09/06/2013
• Issued: 08/20/2019
• Est. Priority Date: 09/14/2012
• Status: Active Grant

• Current Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Original Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Inventors: Pernodet, Nadine A., Dong, Kelly, Pelle, Edward, Yarosh, Daniel B.
• Primary Examiner(s): Alstrum-Acevedo, James H
• Assistant Examiner(s): Martinez, Tara L
• Application Number:
  US14/019,598
• Publication Number:
  US 20150071895A1
• Time in Patent Office: 2,174 Days
• Field of Search: 514 11
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/66 Enzymes
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61K 8/99 from microorganisms other than algae or fungi, e.g. protozoa or bacteria
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

A skin care composition for stimulating selective catabolysis in cells of keratin surfaces comprising at least one autophagy activator and at least one proteasome activator, and a method for improving selective catabolysis in cells of keratin surfaces by treating with the composition.

Claims

1. A composition in the form of an emulsion for improving cellular health and viability of skin cells comprising an autophagy activator in the form of Candida saitoana extract present in an amount ranging from 0.0001 to 5% and a proteasome activator in the form of hydrolyzed algin present in an amount ranging from 0.0001 to 0.01% with all percentages by weight of the total composition wherein the Candida saitoana extract and the hydrolyzed algin in combination are characterized by showing an increase in cellular health and viability when exposed to dermal fibroblasts in vitro when compared to the cellular health and viability of Candida saitoana extract alone and hydrolyzed algin alone.

2. The composition of claim 1 additionally comprising an ingredient selected from the group consisting of:

(a) a peptide having from about 3 to 13 amino acid residues and of the formula (I) (SEQ ID No.

     1);

R₁-(AA)_n-X₁-S-T-P-X₂-(AA)_p-R₂

wherein;

X₁ represents threonine, serine, or is equal to zero,

X₂ represents an isoleucine, leucine, proline, valine, alanine, glycine, or is equal to zero,

AA represents any amino acid or derivative thereof, and n and p are whole numbers between 0 and 4,

R₁ represents the primary amine function of the N-terminal amino acid, either free or substituted by a protective grouping that may be chosen from either an acetyl group, a benzoyl group, a tosyl group, or a benzyloxycarbonyl group,

R₂ represents the hydroxyl group of the carboxyl function of the C-terminal amino acid, which may be substituted by a protective grouping that may be chosen from either a C₁ to C₂₀ alkyl chain or an NH₂, NHY, or NYY group with Y representing a C₁ to C₄ alkyl chain,

wherein said formula (I) sequence may contain substitutions of amino acids X₁ and X₂ with other chemically equivalent amino acids;

(b) Tetrapeptide-26;

(c) cichoric acid; and

(d) mixtures thereof.

3. The composition of claim 1 further comprising a peptide selected from the group consisting of:

S-T-P-NH₂

Ser-Thr-Pro-NH₂

(SEQ ID No.

     2) Y-V-S-T-P-Y-N-NH₂

Tyr-Val-Ser-Thr-Pro-Tyr-Asn-NH₂

(SEQ ID No.

     3) NH₂—

V-S-T-P-E-NH₂

NH₂-Val-Ser-Thr-Pro-Glu-NH₂

(SEQ ID No.

     4) NH₂-L-H-S-T-P-P-NH₂

NH₂-Leu-His-Ser-Thr-Pro-Pro-NH₂

(SEQ ID No.

     5) CH₃NH-R-H-S-T-P-E-NH₂

CH₃-NH-Arg-His-Ser-Thr-Pro-Glu-NH₂

(SEQ ID No.

     6) CH₃NH-H-S-T-P-E-CH₃NH

CH₃—

NH-His-Ser-Thr-Pro-Glu-CH₃—

NH₂

and mixtures thereof.

4. The composition of claim 3 wherein the peptide is selected from:

S-T-P-NH₂

Ser-Thr-Pro-NH₂

(SEQ ID No.

     4) NH₂-L-H-S-T-P-P-NH₂

NH₂-Leu-His-Ser-Thr-Pro-Pro-NH₂

and mixtures thereof.

5. The composition of claim 1 further comprising a peptide which is Tripeptide-32, Tetrapeptide-26 or mixtures thereof.

6. The composition of claim 5 additionally comprising a DNA repair enzyme selected from the group consisting of:

Arabidopsis Thaliana extract either alone or in admixture with lecithin and water;

Lactobacillus ferment,

Micrococcus lysate,

Plankton extract,

Bifida ferment lysate; and

mixtures thereof.

7. The composition of claim 1 wherein the Candida saitoana extract is present in an amount ranging from 0.0001 to 1%.

8. A composition in the form of an emulsion comprising Candida saitoana extract present in an amount ranging from 0.0001 to 5% and a proteasome activator in the form of hydrolyzed algin present in an amount ranging from 0.0001 to 0.01% with all percentages by weight of the total composition wherein the Candida saitoana extract and the hydrolyzed algin in combination are characterized by causing at least a 33% increase in cellular health and viability when exposed to dermal fibroblasts in vitro when compared to the cellular health and viability of Candida saitoana extract alone and hydrolyzed algin alone when exposed to the same cells at the same concentration.