De novo synthesized gene libraries
First Claim
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1. A device for nucleic acid collection, comprising:
- a) a plate, the plate comprising;
a base layer of the plate; and
one or more wells, wherein each well of the one or more wells comprises;
a side wall that extends vertically from the base layer;
an interior region of the well; and
an exterior region of the well,wherein the exterior region of the well has a lower surface energy than the interior region of the well; and
b) a solid support structure, wherein the solid support structure comprises a plurality of clusters, wherein each cluster comprises a plurality of loci, wherein each locus comprises a substantially planar region having a single fluidic opening, wherein each cluster is vertically aligned to a respective well of the plate, wherein the solid support structure comprises nylon, nitrocellulose, polypropylene, silicon, silica, polystyrene, agarose, dextran, cellulosic polymer, polyacrylamide, or polydimethylsiloxane (PDMS), and wherein each cluster of the plurality of clusters has a lower surface energy than the interior region of each well of the plurality of wells of the plate.
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Abstract
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
907 Citations
15 Claims
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1. A device for nucleic acid collection, comprising:
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a) a plate, the plate comprising; a base layer of the plate; and one or more wells, wherein each well of the one or more wells comprises; a side wall that extends vertically from the base layer; an interior region of the well; and an exterior region of the well, wherein the exterior region of the well has a lower surface energy than the interior region of the well; and b) a solid support structure, wherein the solid support structure comprises a plurality of clusters, wherein each cluster comprises a plurality of loci, wherein each locus comprises a substantially planar region having a single fluidic opening, wherein each cluster is vertically aligned to a respective well of the plate, wherein the solid support structure comprises nylon, nitrocellulose, polypropylene, silicon, silica, polystyrene, agarose, dextran, cellulosic polymer, polyacrylamide, or polydimethylsiloxane (PDMS), and wherein each cluster of the plurality of clusters has a lower surface energy than the interior region of each well of the plurality of wells of the plate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A device for nucleic acid collection, comprising:
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a) a plate, the plate comprising; a base layer of the plate; and one or more wells, wherein each well of the one or more wells comprises; a side wall that extends vertically from the base layer; an interior region of the well; and an exterior region of the well, wherein the exterior region of the well has a lower surface energy than the interior region of the well; and b) a solid support structure, wherein the solid support structure comprises a plurality of clusters, wherein each cluster comprises a plurality of loci, wherein each locus comprises a substantially planar region having a single fluidic opening, wherein each cluster is vertically aligned to a respective well of the plate, and wherein each cluster of the plurality of clusters has a lower surface energy than the interior region of each well of the plurality of wells of the plate.
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Specification