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Molecular identity tags and uses thereof in identifying intermolecular ligation products

  • US 10,385,334 B2
  • Filed: 05/30/2014
  • Issued: 08/20/2019
  • Est. Priority Date: 05/31/2013
  • Status: Active Grant
First Claim
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1. A method of identifying intermolecular ligation products, comprising:

  • marking linear target nucleic acids with multiple, mutually distinguishable pairs of molecular identity tags to yield marked target nucleic acids, wherein the marking comprises marking terminal regions of each linear target nucleic acid with one of the pairs of molecular identity tags, wherein a first terminal region of each target nucleic acid is marked with a first member of the one of the pairs and a second terminal region of each target nucleic acid is marked with a second member of the one of the pairs;

    joining terminal ends of the marked target nucleic acids or products of the marked target nucleic acids to yield marked nucleic acid products, each marked nucleic acid product comprising two of the molecular identity tags juxtaposed across the joined terminal ends, wherein the marked nucleic acid products include one or more marked nucleic acid products comprising only juxtaposed paired molecular identity tags and one or more marked nucleic acid products comprising juxtaposed unpaired molecular identity tags, wherein any marked nucleic acid product comprising juxtaposed unpaired molecular identity tags constitutes an intermolecular nucleic acid product; and

    parsing the one or more marked nucleic acid products comprising only juxtaposed paired molecular identity tags, or products thereof, from the one or more marked nucleic acid products comprising juxtaposed unpaired molecular identity tags, or products thereof.

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