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Kinetic exclusion amplification of nucleic acid libraries

  • US 10,385,384 B2
  • Filed: 08/08/2017
  • Issued: 08/20/2019
  • Est. Priority Date: 06/15/2012
  • Status: Active Grant
First Claim
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1. A method for amplifying nucleic acids, comprising(a) providing an amplification reagent comprising(i) an array of amplification sites, and(ii) a solution comprising a plurality of different target nucleic acids,wherein the number of the different target nucleic acids in the solution exceeds the number of amplification sites in the array,wherein the different target nucleic acids have fluidic access to the plurality of amplification sites, andwherein each of the amplification sites comprises a capacity for several nucleic acids in the plurality of different nucleic acids;

  • and(b) reacting the amplification reagent to produce a plurality of amplification sites that each comprise a clonal population of amplicons from an individual target nucleic acid from the solution,wherein the reacting comprises (i) producing a first amplicon from an individual target nucleic acid that transports to each of the amplification sites, and (ii) producing subsequent amplicons from the individual target nucleic acid that transports to each of the amplification sites or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated at the amplification sites exceeds the average rate at which the first amplicon is generated at the amplification sites.

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