Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
First Claim
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1. A method for attaching one or more polynucleotide binding proteins to a target polynucleotide, comprising:
- (a) providing the one or more polynucleotide binding proteins bound to one or more loading moieties; and
(b) covalently attaching the one or more loading moieties to the target polynucleotide.
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Abstract
The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also relates to new methods of characterizing target polynucleotides.
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Citations
20 Claims
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1. A method for attaching one or more polynucleotide binding proteins to a target polynucleotide, comprising:
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(a) providing the one or more polynucleotide binding proteins bound to one or more loading moieties; and (b) covalently attaching the one or more loading moieties to the target polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A kit for covalently attaching one or more polynucleotide binding proteins to a target polynucleotide, comprising (a) the one or more polynucleotide binding proteins bound to one or more loading moieties and (b) a ligase.
Specification