Extraction of lipid from cells and products therefrom

• US 10,392,578 B2
• Filed: 06/01/2011
• Issued: 08/27/2019
• Est. Priority Date: 06/01/2010
• Status: Active Grant

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: Cherinko, Stephen Robert, Kertis, Robert Cody, Apt, Kirk E., Behrens, Paul Warren, Hansen, Jon Milton, Pfeifer, III, Joseph W., Stahl, Tracey Lynn, Zirkle, Ross, Burke, Nasrin Tabayeh, Raman, Krishna, Leininger, Neil Francis
• Primary Examiner(s): Carr, Deborah D
• Application Number:
  US13/151,014
• Publication Number:
  US 20110295028A1
• Time in Patent Office: 3,009 Days
• Field of Search: 554 8, 554223, 554224, 554227, 435134
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11B 3/02 by chemical reaction C11B3/003 takes precedence
• CPC Class Codes: C11B 3/16 by mechanical means
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

Claims

1. An extracted microbial lipid comprising a triglyceride fraction of at least 70% by weight of the lipid fraction, wherein the docosahexaenoic acid content of the triglyceride fraction is at least 50% by weight, wherein the docosapentaenoic acid n-6 content of the triglyceride fraction is from at least 0.5% by weight to 6% by weight, and wherein the oil has an anisidine value of 0.5 to 26, and wherein the lipid has less than 5% by weight of an organic solvent.

4. The extracted microbial lipid of claim 1, wherein the oil has a peroxide value of 5 or less.

5. The extracted microbial lipid of claim 1, wherein the oil has a phosphorus content of 100 ppm or less.

6. The extracted microbial lipid of claim 1, wherein the lipid is a crude oil.

7. A process for obtaining a lipid, the process comprising refining a crude lipid of claim 6.

2. An extracted microbial lipid comprising a triglyceride fraction of at least 70% by weight of the lipid fraction, wherein the docosahexaenoic acid content of the triglyceride fraction is at least 40% by weight, wherein the docosapentaenoic acid n-6 content of the triglyceride fraction is from at least 0.5% by weight to 6% by weight, wherein the ratio of docosahexaenoic acid to docosapentaenoic acid n-6 is greater than 6:

1, and wherein the oil has an anisidine value of 0.5 to 26, and wherein the lipid has less than 5% by weight of an organic solvent.

3. An extracted microbial lipid comprising a triglyceride fraction of at least about 70% by weight, of the lipid fraction wherein the docosahexaenoic acid content of the triglyceride fraction is at least 60% by weight and wherein the oil has an anisidine value of 0.5 to 26, and wherein the lipid has less than 5% by weight of an organic solvent.