Cartridge and apparatus for preparing a biological sample

• US 10,393,634 B2
• Filed: 09/24/2013
• Issued: 08/27/2019
• Est. Priority Date: 12/13/2012
• Status: Active Grant

• Current Assignee: Koninklijke Philips N.V.
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Wimberger-Friedl, Reinhold, Van Hemert, Freek, Van De Stolpe, Anja, Prins, Menno Willem Jose, Van Tooren, Johannes Adrianus
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US14/651,859
• Publication Number:
  US 20150316454A1
• Time in Patent Office: 2,163 Days
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/082 Active control of flow resistance, e.g. flow controllers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates

Abstract

A cartridge and an associated apparatus for preparing a biological sample includes a reaction chamber configured to be closed by a substrate carrying the biological sample, where the reaction chamber is connected to a fluidic inlet system and a fluidic outlet system. When the substrate closes the reaction chamber, the fluidic inlet system, the reaction chamber, and the fluidic outlet system constitute a fluidic system that is closed to an environment outside the cartridge with respect to an exchange of liquids. Further, a pressure-driven flow through the reaction chamber may be induced by actuation of deformable membranes formed in the cartridge.

Claims

1. A cartridge for processing a sample carried on a substrate, the cartridge comprising:

a cartridge body comprising;

a reaction chamber;

walls that surround the reaction chamber and define an opening extending between the walls and across the reaction chamber;

a fluidic inlet system configured to provide at least one reagent fluid to the reaction chamber;

a fluidic outlet system configured to receive fluid from the reaction chamber; and

corresponding pump membranes positioned in the fluidic inlet system and the fluidic outlet system, wherein the corresponding pump membranes are configured to induce a pressure-driven fluid flow from the fluidic inlet system through the reaction chamber to the fluidic outlet system;

wherein the fluidic inlet system, the reaction chamber, and the fluidic outlet system are configured to be closed to an exchange of liquids with an environment outside the cartridge by the walls of the cartridge body and a wall of the substrate carrying the sample in response to an attachment of the substrate carrying the sample over the walls of the cartridge body and the opening.

2. The cartridge according to claim 1, wherein the wall of the substrate comprises a flat surface and the opening of the reaction chamber is configured to be closed by the flat surface with at least a portion of the flat surface being positioned inside an interior of the reaction chamber after the attachment of the substrate carrying the sample.

3. The cartridge according to claim 1, wherein the cartridge body comprises at least one of an adhesive, a glue, an intermediate vacuum chamber, and a source of electromagnetic forces, configured to provide the attachment of the substrate carrying the sample.

4. The cartridge according to claim 1, wherein a height of the reaction chamber above the substrate carrying the sample closing the opening is less than about 50 μ

m.

5. The cartridge according to claim 1, further comprising spacers disposed between the substrate carrying the sample and the cartridge body, with each spacer spaced apart from each other spacer.

6. The cartridge according to claim 1, wherein the corresponding pump membranes are configured to be at least one of moved and deformed to induce the pressure-driven fluid flow.

7. The cartridge according to claim 1, wherein the fluidic inlet system comprises at least one reagent reservoir filled with the at least one reagent fluid, wherein said reservoir is sealed from the environment outside the cartridge.

8. The cartridge according to claim 1, further comprising at least one of a marker configured to be localized by an external detector and an identifier configured to be identified by an external detector.

9. The cartridge according to claim 1, further comprising at least one indicator configured to indicate a status of at least one of a reagent and a reaction.

10. The cartridge according to claim 1, further comprising a control element configured to route a fluid from the fluidic inlet system through the reaction chamber to the fluidic outlet system.

11. The cartridge according to claim 1, wherein at least one of the fluidic inlet system and the fluidic outlet system is completely arranged within an interior of the cartridge body between a top surface and a bottom surface of the cartridge body.

12. The cartridge according to claim 1, wherein the corresponding pump membranes are configured to be at least one of moved and deformed to induce the pressure-driven fluid flow.

13. A cartridge for processing a sample carried on a substrate, the cartridge comprising:

a cartridge body comprising;

a reaction chamber;

walls that surround the reaction chamber and define an opening;

a fluidic inlet system configured to provide at least one reagent fluid to the reaction chamber;

a fluidic outlet system configured to receive fluid from the reaction chamber; and

corresponding pump membranes positioned in the fluidic inlet system and the fluidic outlet system, wherein the corresponding pump membranes are configured to induce a pressure-driven fluid flow from the fluidic inlet system through the reaction chamber to the fluidic outlet system, and

wherein the fluidic inlet system, the reaction chamber, and the fluidic outlet system are configured to be closed to an exchange of liquids with an environment outside the cartridge by the walls of the cartridge body and a wall of the substrate carrying the sample in response to an attachment of the substrate carrying the sample over the opening.