Mutant CsgG pores
First Claim
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1. An apparatus for characterizing a target analyte, the apparatus comprising a mutant CsgG monomer comprising a variant of the sequence shown in SEQ ID NO:
- 390, wherein the variant comprises a mutation at one or more of positions Y51, N55 and F56, and an in vitro membrane, wherein the monomer is inserted into the in vitro membrane.
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Abstract
The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
132 Citations
8 Claims
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1. An apparatus for characterizing a target analyte, the apparatus comprising a mutant CsgG monomer comprising a variant of the sequence shown in SEQ ID NO:
- 390, wherein the variant comprises a mutation at one or more of positions Y51, N55 and F56, and an in vitro membrane, wherein the monomer is inserted into the in vitro membrane.
- View Dependent Claims (2, 3, 4, 5)
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6. An apparatus for characterizing a target analyte, the apparatus comprising a plurality of mutant CsgG monomers, each monomer comprising a variant of the sequence shown in SEQ ID NO:
- 390, wherein each variant comprises a mutation at one or more of positions Y51, N55 and F56, and wherein the plurality of monomers is inserted into a plurality of in vitro membranes.
- View Dependent Claims (7, 8)
Specification
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Current AssigneeOxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit Brussel
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Original AssigneeOxford Nanopore Technologies Limited
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InventorsHoworka, Stefan, Remaut, Han, Jayasinghe, Lakmal, Wallace, Elizabeth Jayne, Clarke, James Anthony, Hambley, Richard George, Pugh, Jonathan Bankes
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Primary Examiner(s)Carlson, Karen Cochrane
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Application NumberUS15/507,947Publication NumberTime in Patent Office1,463 DaysField of SearchUS Class CurrentCPC Class CodesC07K 14/245 Escherichia (G)C07K 2319/22 containing a Strep-tagC12Q 1/68 involving nucleic acidsC12Q 1/6869 Methods for sequencingC12Q 1/6874 involving nucleic acid arra...C12Q 2565/631 being a biochannel or poreG01N 27/447 using electrophoresis
