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Label-free detection of small and large molecule interactions, and activities in biological systems

  • US 10,401,298 B2
  • Filed: 01/23/2018
  • Issued: 09/03/2019
  • Est. Priority Date: 12/15/2014
  • Status: Active Grant
First Claim
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1. A system for quantitative detection and analysis of the binding of molecules with molecular receptors on the surfaces of a biological object, the system comprising:

  • a microscope positioned to image the biological object and transmit a time sequence of images of the biological object;

    a camera located to receive the transmitted time sequence of images of the biological object from the microscope and transmit data representing the time sequence of images;

    a processor, coupled to receive the data representing the time sequence of images from the camera;

    the processor including an image processing algorithm that quantitatively determines mechanical deformation of the biological object from the time sequence of images, wherein the mechanical deformation is due to an interaction of the biological object with a plurality of molecules, wherein the image processing algorithm determines mechanical deformation by using an edge detector to determine an edge of the biological object and a time sequence tracker for tracking a plurality of movements of the edge by measuring positions of the edge at different times, and wherein the image processing algorithm also determines molecular binding kinetic values of the biological object as proportional to the mechanical deformation of the biological object.

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