Devices and methods for sample characterization
First Claim
Patent Images
1. A method, comprising:
- applying an electric field across a separation channel in a microfluidic device to effect a separation of an analyte mixture via isoelectric focusing;
simultaneously and continuously imaging the separation and mobilization of the separated analyte mixture in the whole separation channel or a portion thereof; and
expelling separated and mobilized analytes via electrospray ionization from an orifice on the microfluidic device into a mass spectrometer.
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Abstract
Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps.
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Citations
26 Claims
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1. A method, comprising:
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applying an electric field across a separation channel in a microfluidic device to effect a separation of an analyte mixture via isoelectric focusing; simultaneously and continuously imaging the separation and mobilization of the separated analyte mixture in the whole separation channel or a portion thereof; and expelling separated and mobilized analytes via electrospray ionization from an orifice on the microfluidic device into a mass spectrometer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method, comprising:
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performing a separation of an analyte mixture in a separation channel in a microfluidic device; simultaneously and continuously imaging the separation and mobilization of the separated analyte mixture in the whole separation channel or a portion thereof; expelling separated and mobilized analytes via electrospray ionization from an orifice on the microfluidic device into a mass spectrometer; and correlating separated analyte peaks detected in the separation channel with mass spectrometer data for the separated analytes. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification