Reagent preparation assembly

• US 10,406,524 B2
• Filed: 01/15/2015
• Issued: 09/10/2019
• Est. Priority Date: 06/29/2010
• Status: Active Grant

• Current Assignee: Biolyph, LLC
• Original Assignee: Biolyph, LLC
• Inventors: Pearcy, Timothy, Skakoon, James G.
• Primary Examiner(s): Sorkin, David L
• Application Number:
  US14/597,677
• Publication Number:
  US 20150125364A1
• Time in Patent Office: 1,699 Days
• Field of Search: 206222, 604 87, 604 88
• CPC Class Codes: B01F 33/50112 of the syringe or cartridge type
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/105 using desiccants
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: Y10T 29/49826 Assembling or joining

Abstract

A reagent preparation assembly includes a body and a reaction chamber adjacent the body, the reaction chamber includes a reagent therein, such as a lyophilized reagent. An access port extends into the reaction chamber, and the access port is configured to receive an instrument. A seal extends across a portion of the reaction chamber and the access port. A reconstitution assembly is movably coupled with the body. The reconstitution assembly includes a plunger, a syringe and a piston. The plunger is movably coupled with the body. The syringe is selectively engaged with the plunger. The syringe includes a solution reservoir containing a solution, and movement of the syringe pierces the seal. The piston is selectively engaged with the plunger, and the piston is movably coupled within the syringe. Movement of the piston pushes the solution into the reaction chamber.

Claims

1. A reagent preparation assembly comprising:

a reaction chamber, the reaction chamber includes an unreconstituted reagent therein;

an access port extending toward the reaction chamber, the access port is configured to receive an instrument;

a body coupled with the reaction chamber;

a syringe passage within the body extending toward the reaction chamber and a syringe port, the syringe passage is isolated from the reaction chamber with a syringe seal; and

a reconstitution assembly coupled with the body, the reconstitution assembly includes a plunger movable between initial, piercing and solution moving positions;

in the initial position a syringe is suspended above the syringe seal and the reaction chamber, the syringe includes a solution reservoir containing a solution,

in the piercing position the plunger is moved and the plunger drives the syringe through the syringe seal, and

in the solution moving position the plunger is moved relative to the piercing position, the plunger drives a piston within the syringe, and the piston pushes solution from the solution reservoir of the syringe into the reaction chamber.

2. The reagent preparation assembly of claim 1, wherein in the piercing position the plunger is engaged with the syringe and in the liquid moving position the plunger is engaged with the piston.

3. The reagent preparation assembly of claim 2, wherein in the liquid moving position the plunger is disengaged with the syringe.

4. The reagent preparation assembly of claim 2, wherein a deflectable tongue engages the plunger with the syringe in the piercing position and the deflectable tongue is disengaged with the syringe in liquid moving position.

5. The reagent preparation assembly of claim 4, wherein the body includes a camming surface, and movement of the plunger between the piercing position and the liquid moving position moves the deflectable tongue over the camming surface to disengage the deflectable tongue from the syringe.

6. The reagent preparation assembly of claim 1, wherein in the liquid moving position at least a portion of the syringe including a syringe orifice in communication with the solution reservoir is within the reaction chamber.

7. The reagent preparation assembly of claim 1, wherein the reaction chamber tapers from a first location underlying the syringe to a second location underlying the access port.

8. The reagent preparation assembly of claim 1, wherein the plunger continuously moves from the initial position to the piercing position and from the piercing position to the solution moving position with depression of the plunger.