Hardware for magnetic 3D culture

• US 10,407,660 B2
• Filed: 02/07/2013
• Issued: 09/10/2019
• Est. Priority Date: 08/10/2010
• Status: Active Grant

• Current Assignee: Greiner Bio-One North America Inc. (Greiner AG)
• Original Assignee: Greiner Bio-One North America Inc. (Greiner AG)
• Inventors: Souza, Glauco
• Primary Examiner(s): Hassan, Liban M
• Application Number:
  US13/762,103
• Publication Number:
  US 20140220672A1
• Time in Patent Office: 2,406 Days
• Field of Search: 4353053, 4352891, 4353081, 4351731, 4352831, 4353054, 29428, 29453
• CPC Class Codes: B01L 2200/0657 Pipetting powder
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/0289 Apparatus for withdrawing or distributing predetermined quantities of fluid B01L3/02 takes precedence; sample taking G01N1/00; sample taking within automatic analysers G01N35/00; volume measuring in general G01F
• CPC Class Codes: C12M 23/10 Petri dish crystallising dishes B01L3/06
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02
• CPC Class Codes: C12M 35/06 Magnetic means C12M35/02 takes precedence
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02

Abstract

Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats.

Claims

1. A magnetic cell culture system, comprising

a cell culture vessel, and a cap shaped to fit over or under said cell culture vessel, said cap including a lip around an outer edge of said cap, wherein said lip fits over and surrounding an outer edge of said cell culture vessel, said cell culture vessel having one or more wells, and said cap having one or more depressions on said cap corresponding to a center of the one or more wells, wherein one or more magnets are affixed to said cap inside said one or more depressions such that said one or more magnets sit above or below said cell culture vessel and do not touch a media in said cell culture vessel,

wherein said cap comprises magnet position adjusters configured to adjust the position of said one or more magnets affixed into said one or more depressions in two or more of the three spatial directions, wherein the position of said one or more magnets can mechanically, electrically and/or magnetically be adjusted in said two or more of the three spatial directions, and wherein the cell culture vessel is a Petri dish, a cell culture flask, or a multiwell culturing plate.

2. The magnetic cell culture system of claim 1, wherein one of said magnet position adjusters of said cap is configured to adjust a height of said one or more magnets from said cell culture vessel.

3. The magnetic cell culture system of claim 1, wherein said cap is disc shaped, and wherein one of said magnet position adjusters of said cap includes a screwable collar that fits inside or outside said cap, so that unscrewing said collar functions to raise said cap from said cell culture vessel to a level and maintain said cap at the level while horizontally moving said one or more magnets by a second magnet position adjuster of said cap.

4. The magnetic cell culture system of claim 1, wherein said one or more magnets are reversibly fit into said one or more depressions.

5. The magnetic cell culture system of claim 1, wherein said cell culture vessel has multiple wells, and said one or more magnets are affixed to said cap at a center of each well of said multiple wells so that each well has a magnet.

6. The magnetic cell culture system of claim 1, wherein said one or more magnets are snap fit into said one or more depressions.

7. The magnetic cell culture system of claim 1, wherein said one or more magnets are adhered into said one or more depressions.

8. A magnetic cell culture vessel, comprising a vessel having a plurality of wells in an array, a cap covering said plurality of wells, said cap having a lip surrounding an outer edge of said vessel and a plurality of magnets in an array affixed to said cap, such that each well of said plurality of wells has a magnet of said plurality of magnets over or under said well at a center of said well and said magnet does not touch a media in said well when said cap sits in place over or under said vessel, wherein said cap comprises magnet position adjusters configured to adjust the position of said one or more magnets affixed into said one or more depressions in two or more of the three spatial directions, wherein the position of said one or more magnets can mechanically, electrically and/or magnetically be adjusted in the two or more of the three spatial directions, and wherein the vessel is a Petri dish, cell culture flask, or a multiwell culturing plate.

9. The magnetic cell culture vessel of claim 8, wherein said array of wells is an array of 6, 8, 12, 96, 384, or 1536 wells.

10. The magnetic cell culture vessel of claim 8, including a second cap that fits between said vessel and said cap.

11. A magnetic drive, comprising a cap sized to fit over or under an ANSI/SLAS microtiter plate having a plurality of wells,

said cap having a lip around an outer edge thereof, said lip fitting around an outside edge of said ANSI/SLAS microtiter plate, said cap having a plurality of depressions having a plurality of magnets affixed thereinto, such that each well of said plurality of wells has a magnet of said plurality of magnets over or under said well at a center of said well when said magnetic drive is disposed over or under said ANSI/SLAS microtiter plate such that said magnet does not touch a media in said well, and wherein said cap comprises magnet position adjusters configured to adjust the position of said one or more magnets affixed into said one or more depressions in two or more of the three spatial directions, wherein the position of said one or more magnets can mechanically, electrically and/or magnetically be adjusted in said two or more of the three spatial directions.

12. The magnetic drive of claim 11, wherein each of said magnets having a Brmax of at least 12600 Gauss.