Diagnosis of melanoma by nucleic acid analysis
First Claim
Patent Images
1. A method for detecting an expression level of preferentially expressed antigen in melanoma (PRAME) in a subject, the method comprising:
- (a) obtaining a biological sample of a skin lesion suspected of comprising melanoma from the subject with an adhesive tape, wherein the biological sample comprises or is suspected of comprising a nucleic acid molecule expressed from preferentially expressed antigen in melanoma (PRAME), and wherein the biological sample comprises cells from the stratum corneum; and
(b) detecting whether the expression level of preferentially expressed antigen in melanoma (PRAME) in the biological sample is at a level that is above an expression level of PRAME in a non-melanoma sample by application of a detectably labeled probe that hybridizes to a nucleic acid molecule expressed from PRAME, whereby the presence of a nucleic acid molecule expressed from PRAME in the biological sample in an amount that is greater than the presence of PRAME in a non-melanoma sample is indicative of melanoma in the biological sample.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides methods for diagnosing melanoma and/or solar lentigo in a subject by analyzing nucleic acid molecules obtained from the subject. The present invention also provides methods for distinguishing melanoma from solar lentigo and/or dysplastic nevi and/or normal pigmented skin. The methods include analyzing expression or mutations in epidermal samples, of one or more skin markers. The methods can include the use of a microarray to analyze gene or protein profiles from a sample.
124 Citations
14 Claims
-
1. A method for detecting an expression level of preferentially expressed antigen in melanoma (PRAME) in a subject, the method comprising:
-
(a) obtaining a biological sample of a skin lesion suspected of comprising melanoma from the subject with an adhesive tape, wherein the biological sample comprises or is suspected of comprising a nucleic acid molecule expressed from preferentially expressed antigen in melanoma (PRAME), and wherein the biological sample comprises cells from the stratum corneum; and (b) detecting whether the expression level of preferentially expressed antigen in melanoma (PRAME) in the biological sample is at a level that is above an expression level of PRAME in a non-melanoma sample by application of a detectably labeled probe that hybridizes to a nucleic acid molecule expressed from PRAME, whereby the presence of a nucleic acid molecule expressed from PRAME in the biological sample in an amount that is greater than the presence of PRAME in a non-melanoma sample is indicative of melanoma in the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A method for detecting melanoma in a biological sample, comprising:
-
(a) obtaining a biological sample of a skin lesion suspected of comprising melanoma from a subject with an adhesive tape;
wherein the biological sample comprises or is suspected of comprising a nucleic acid molecule expressed from preferentially expressed antigen in melanoma (PRAME), wherein the nucleic acid molecule comprises an RNA molecule, andwherein the biological sample comprises cells from the stratum corneum; (b) generating a complementary DNA (cDNA) product from the RNA molecule; (c) detecting an expression level of the nucleic acid molecule expressed from preferentially expressed antigen in melanoma (PRAME) in the biological sample by performing real-time quantitative PCR to amplify and quantify the cDNA product; and (d) comparing the expression level of the nucleic acid molecule expressed from PRAME based on quantification of the cDNA product in the biological sample to the expression level of a corresponding nucleic acid molecule expressed from PRAME in a non-melanoma sample, whereby an increased expression level of the nucleic acid molecule expressed from PRAME in the biological sample as compared to the non-melanoma sample is indicative of melanoma, thereby detecting melanoma in the biological sample of a skin lesion. - View Dependent Claims (12, 13, 14)
-
Specification