Cell capture system and method of use
First Claim
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1. A system comprising:
- a substrate defining a set of chambers defined at a broad surface, wherein each chamber in the set of chambers is configured to retain a respective single particle of a set of particles and comprises;
a set of walls defining a chamber volume, andan open surface permitting access of the respective single particle to the chamber volume from a direction perpendicular to the broad surface of the substrate,wherein the set of walls of each chamber of the set of chambers fluidly isolates adjacent chambers of the set of chambers, and wherein fluid received at the inlet channel enters each chamber only by way of the open surface of each chamber; and
an inlet channel positioned superior the set of chambers and directly fluidly coupled to the set of chambers, whereby fluid received at the inlet channel is configured to concurrently access each of the chambers in the set of chambers at the open surface of each chamber, and wherein the system is configured to receive a distribution of a reagent fluid in a direction substantially parallel to the broad surface through the inlet channel and to permit access of the reagent fluid to the set of chambers.
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Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
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Citations
20 Claims
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1. A system comprising:
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a substrate defining a set of chambers defined at a broad surface, wherein each chamber in the set of chambers is configured to retain a respective single particle of a set of particles and comprises; a set of walls defining a chamber volume, and an open surface permitting access of the respective single particle to the chamber volume from a direction perpendicular to the broad surface of the substrate, wherein the set of walls of each chamber of the set of chambers fluidly isolates adjacent chambers of the set of chambers, and wherein fluid received at the inlet channel enters each chamber only by way of the open surface of each chamber; and an inlet channel positioned superior the set of chambers and directly fluidly coupled to the set of chambers, whereby fluid received at the inlet channel is configured to concurrently access each of the chambers in the set of chambers at the open surface of each chamber, and wherein the system is configured to receive a distribution of a reagent fluid in a direction substantially parallel to the broad surface through the inlet channel and to permit access of the reagent fluid to the set of chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system comprising:
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a substrate defining a set of chambers defined at a broad surface, wherein each chamber in the set of chambers is configured to retain a respective single particle of a set of particles and comprises; a set of walls defining a chamber volume and configured to provide physical isolation from adjacent chambers, and an open surface permitting access to the chamber volume from a direction perpendicular to the broad surface of the substrate, wherein fluid received at the inlet channel enters each chamber only by way of the open surface of each chamber; and an inlet channel positioned superior the set of chambers and directly fluidly coupled to the set of chambers, whereby fluid received at the inlet channel is configured to be distributed across each of the chambers in the set of chambers and enter the open surface of each chamber. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification