Process, arrangements and systems for providing frequency domain imaging of a sample

• US 10,413,175 B2
• Filed: 06/08/2016
• Issued: 09/17/2019
• Est. Priority Date: 05/10/2006
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Yun, Seok-Hyun, De Boer, Johannes F.
• Primary Examiner(s): Brutus, Joel F
• Application Number:
  US15/176,938
• Publication Number:
  US 20160367132A1
• Time in Patent Office: 1,196 Days
• Field of Search: None
• CPC Class Codes: A61B 3/0025 characterised by electronic signal processing, e.g. eye models
• CPC Class Codes: A61B 3/102 for optical coherence tomography [OCT]
• CPC Class Codes: A61B 3/1233 for measuring blood flow, e.g. at the retina
• CPC Class Codes: A61B 3/14 Arrangements specially adapted for eye photography apparatus or arrangements for taking photographs per se G03B
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/418 lymph vessels, ducts or nodes
• CPC Class Codes: G01N 21/45 using interferometric methods; using Schlieren methods
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

Exemplary apparatus, arrangement and method can be provided for obtaining information associated with an anatomical structure or a sample using optical microscopy. For example, a light radiation can be separated into first light radiation(s) directed to an anatomical sample and second light radiation directed to a reference. A wavelength of the radiation can vary over time, and the wavelength can be shorter than approximately 1150 nm. An interference can be detected between third and fourth radiations associated with the first and second radiations and fourth radiation, respectively. At least one image corresponding to portion(s) of the sample can be generated using data associated with the interference. In addition, source(s) can be provided which can be configured to provide an electromagnetic radiation having a wavelength that varies over time. A period of a variation of the wavelength of the first electromagnetic radiation(s) can be shorter than 1 millisecond, and the wavelength can be shorter than approximately 1150 nm.

Claims

1. An apparatus comprising:

at least one light source configured to provide a light radiation;

a splitter configured to separate the light radiation into at least one first electro-magnetic radiation directed to an anatomical sample and at least one second electro-magnetic radiation directed to a reference, wherein the anatomical sample includes at least one section, and wherein a wavelength of the light radiation provided by the at least one light source varies over time, and the wavelength is shorter than approximately 1150 nm;

at least one interferometer configured to interfere at least one third radiation associated with the at least one first radiation and at least one fourth radiation associated with the at least one second radiation to generate an interference signal;

at least one detector configured to detect the interference signal; and

at least one computer configured to generate at least one image corresponding to at least one portion of the anatomical sample using data associated with the interference signal.

2. The apparatus according to claim 1, wherein a period of a variation of the wavelength of the at least one first electro-magnetic radiation by the at least one light source is shorter than 1 millisecond.

3. The apparatus according to claim 1, wherein the at least one section includes at least one of a retina, a choroid, an optic nerve, or a fovea.

4. The apparatus according to claim 1, wherein the wavelength is shorter than approximately 950 nm.

5. The apparatus according to claim 1, wherein the wavelength is varied with the at least one light source by at least 10 nm over a period of a variation of the wavelength of the at least one first electro-magnetic radiation.

6. The apparatus according to claim 1, further comprising at least one scanner which is configured to scan the at least one first electro-magnetic radiation laterally across the anatomical sample.

7. The apparatus according to claim 1, wherein the anatomical sample has an anatomical structure and the at least one image is associated with the anatomical structure of the anatomical sample.

8. The apparatus according to claim 7, wherein the at least one image is further associated with at least one of a blood or a lymphatic flow in the anatomical sample.

9. The apparatus according to claim 1, wherein the at least one computer is configured and specifically programmed to (i) obtain at least one signal associated with at least one phase of at least one frequency component of the interference signal over less than an entire sweep of the wavelength, and (ii) compare the at least one phase to at least first information.

10. The apparatus according to claim 9, wherein the first information is at least one of (i) associated with a further signal obtained from a sweep of the wavelength that is different from the sweep of the wavelength of the at least one signal, (ii) a constant, or (iii) associated with at least one phase of at least one further frequency component of the interference signal over less than an entire sweep of the wavelength, and wherein the at least one frequency component and the at least one further frequency component are different from one another.

11. The apparatus according to claim 9, further comprising an optical configuration configured to receive one or more of the at least one first electro-magnetic radiation and the at least one second electro-magnetic radiation, and providing at least one fifth electro-magnetic radiation associated with the at least one first electro-magnetic radiation or the at least one second electro-magnetic radiation, wherein the at least one detector is further configured to detect a further interference signal between the at least one fifth radiation and the at least one fourth radiation, and wherein the at least one detector is further configured to obtain at least one reference signal associated with a further phase of at least one first frequency component of the further interference signal over less than an entire sweep of the wavelength.

12. The apparatus according to claim 11, wherein the first information is the further phase.

13. The apparatus according to claim 1, wherein the at least one computer is further configured and programmed to generate a two-dimensional fundus-type reflectivity profile of the anatomical sample.

14. The apparatus according to claim 1, wherein the at least one computer is further configured and programmed to generate a two-dimensional fundus-type image of the anatomical sample based the at least one signal.

15. The apparatus according to claim 1, wherein the wavelength of the radiation provided by the at least one light source that varies over time is swept in a controllable manner.

16. The apparatus according to claim 1, wherein the detector includes at least one single detector which detects the interference signal over multiple different wavelengths that change over time.

17. A method comprising:

causing a transmission of a light radiation;

causing a separation of the light radiation into at least one first electro-magnetic radiation directed to be provided to an anatomical sample and at least one second electro-magnetic radiation directed to a reference, wherein the anatomical sample includes at least one section, and wherein a wavelength of the radiation varies over time, and the wavelength is shorter than approximately 1150 nm;

causing an interference of at least one third radiation associated with the at least one first radiation and at least one fourth radiation associated with the at least one second radiation to generate an interference signal;

detecting the interference signal; and

generating at least one image corresponding to at least one portion of the anatomical sample using data associated with the interference signal.

18. A software arrangement provided on a non-transitory computer-accessible medium and executable by a computer, the computer-accessible medium comprising:

a first set of instructions which, when executed by the computer, controls at least one first electro-magnetic radiation to be directed to be provided to an anatomical sample and at least one second electro-magnetic radiation to be directed to a reference, wherein the anatomical sample includes at least one section, and wherein a wavelength of the radiation varies over time, and the wavelength is shorter than approximately 1150 nm;

a second set of instructions which, when executed by the computer, causes a detection of an interference between at least one third radiation associated with the at least one first radiation and at least one fourth radiation associated with the at least one second radiation using a detector to generate an interference signal; and

a second set of instructions which, when executed by the computer, causes the computer to generate at least one digital image corresponding to at least one portion of the anatomical sample using data associated with the interference signal.

19. The apparatus according to claim 18, wherein the detector includes at least one single detector which detects the interference signal over multiple different wavelengths that change over time.

20. An apparatus comprising:

at least one computer configured to receive first data for a three-dimensional image of at least one portion of a sample which includes at least one section,

wherein the first data is associated with an optical interferometric signal generated from light signals obtained from the anatomical sample and a reference, wherein the optical interferometric signal is based on a radiation whose a wavelength provided from a light source varies over time,

wherein the at least one computer is further configured to convert a region that is less than an entire portion of the first data to second digital data to generate a digital two-dimensional image which is associated with the at least one portion of the anatomical sample,

wherein the at least one computer is still further configured to automatically select the region based on at least one characteristic of the anatomical sample, and

wherein the entire portion is associated with an internal structure within the anatomical sample.

21. An apparatus comprising:

at least one light source configured to provide a light radiation;

a splitter configured to separate the light radiation into at least one first electro-magnetic radiation directed to an anatomical sample and at least one second electro-magnetic radiation directed to a reference, wherein a wavelength of the radiation provided by the at least one light source varies over time, and wherein the anatomical sample includes at least one section; and

at least one interferometer configured to interfere at least one third radiation associated with the at least one first radiation and at least one fourth radiation associated with the at least one second radiation to generate an interference signal;

at least one detector configured to detect the interference signal, wherein the at least one detector is configured to obtain at least one signal associated with at least one phase of at least one frequency component of the interference signal over less than an entire sweep of the wavelength; and

a computer configured to compare the at least one phase to at least first information.

22. The apparatus according to claim 21, wherein the wavelength of the radiation provided by the at least one light source that varies over time is swept in a controllable manner.