Nanostructures for modulating intercellular communication and uses thereof

• US 10,413,565 B2
• Filed: 10/31/2016
• Issued: 09/17/2019
• Est. Priority Date: 04/30/2014
• Status: Active Grant

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Plebanek, Michael P., Thaxton, C. Shad, Mutharasan, Raja Kannan, Angeloni, Nicholas L., McMahon, Kaylin M.
• Primary Examiner(s): Park, H. Sarah
• Application Number:
  US15/339,500
• Publication Number:
  US 20170087094A1
• Time in Patent Office: 1,051 Days
• CPC Class Codes: A61K 31/713 Double-stranded nucleic acids or oligonucleotides
• CPC Class Codes: A61K 47/6425 the peptide or protein in the drug conjugate being a receptor, e.g. CD4, a cell surface antigen, i.e. not a peptide ligand targeting the antigen, or a cell surface determinant, i.e. a part of the surface of a cell
• CPC Class Codes: A61K 47/6917 the form being a lipoprotein vesicle, e.g. HDL or LDL proteins

Abstract

Nanostructures, compositions and methods for treating vesicle-related or exosome-related conditions are provided. In some cases, the nanostructures and/or compositions may be used to treat cancers, neurological disorders, rheumatologic disorders, viral disorders or other diseases or conditions at least in part by regulating vesicle uptake. Methods of analyzing, imaging and modulating vesicles and cellular vesicles processes are also provided.

Claims

1. A synthetic nanostructure vesicle complex isolated from a biological sample or a tissue culture medium comprising

a vesicle, the vesicle having a surface-bound receptor, wherein the vesicle is an exosome, and wherein the surface-bound receptor is SR-B1, and

a synthetic nanostructure,

wherein the synthetic nanostructure is bound to the surface-bound receptor.

2. The complex of claim 1, wherein the synthetic nanostructure comprises a nanostructure core,

a shell, the shell comprising a lipid layer surrounding and attached to the nanostructure core, and

a protein associated with the shell.

3. The complex of claim 1, wherein the synthetic nanostructure further comprises a diagnostic agent.

4. The complex of claim 3, wherein the diagnostic agent is a tracer lipid.

5. The complex of claim 4, wherein the tracer lipid comprises a chromophore, a biotin subunit, or both a chromophore and a biotin subunit.

6. The complex of claim 1, wherein the synthetic nanostructure further comprises a therapeutic agent.

7. The complex of claim 6, wherein the therapeutic agent is a nucleic acid, antiviral agent, antineurological agent, or antirheumatologic agent.