Paint having cell wall particulate material with a protective organophosphorus esterase

• US 10,413,769 B2
• Filed: 07/09/2015
• Issued: 09/17/2019
• Est. Priority Date: 09/09/2002
• Status: Expired due to Term

• Current Assignee: Reactive Surfaces Limited
• Original Assignee: Reactive Surfaces Limited
• Inventors: McDaniel, C. Steven
• Primary Examiner(s): Swope, Sheridan
• Application Number:
  US14/795,608
• Publication Number:
  US 20150328490A1
• Time in Patent Office: 1,531 Days
• Field of Search: None
• CPC Class Codes: A62D 2101/02 Chemical warfare substances, e.g. cholinesterase inhibitors
• CPC Class Codes: A62D 2101/26 containing nitrogen or phosphorus
• CPC Class Codes: A62D 3/02 by biological methods, i.e. processes using enzymes or microorganisms
• CPC Class Codes: A62D 5/00 Composition of materials for coverings or clothing affording protection against harmful chemical agents
• CPC Class Codes: C09D 5/00 Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced electrically insulating plastics, resins or waxes H01B3/30; Filling pastes
• CPC Class Codes: C09D 5/34 Filling pastes materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12
• CPC Class Codes: C09D 7/48 Stabilisers against degradation by oxygen, light or heat
• CPC Class Codes: C09J 11/02 Non-macromolecular additives
• CPC Class Codes: C12N 11/16 Enzymes or microbial cells immobilised on or in a biological cell
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)

Abstract

Disclosed herein are novel coatings and paints comprising a biomolecule composition, wherein the biomolecule composition comprises a phosphoric triester hydrolase. Also disclosed herein are methods of detoxification of a surface contaminated with an organophosphorus compound by contacting the surface with such a coating or paint. Also disclosed herein are novel coating and paint components derived from microorganisms.

Claims

1. A paint comprising an enzymatically active esterase classified in an enzyme subclass designated by Enzyme Commission number EC 3.1.8.1;

wherein the paint comprises a cell wall material;

wherein the enzymatically active esterase comprises the aryldialkylphosphatase encoded by the gene set forth by Genbank accession number M20392 or M22863 or an enzymatically active fragment of said aryldialkylphosphatase;

wherein the aryldialkylphosphatase or fragment has catalytic activity for at least 2months after the paint has undergone solid, adherent film formation on a surface.

2. The paint of claim 1, wherein the cell wall material is in the form of a cell fragment microorganism based particulate material.

3. The paint of claim 1, wherein the enzymatically active esterase comprises a Co²⁺, Fe²⁺, Cu²⁺, Mn²⁺, Cd^2+, or Ni²+at the enzyme active site.

4. The paint of claim 1, wherein the paint comprises one or more of a binder, a liquid component, a colorant, an additive, or a combination thereof.

5. The paint of claim 4, wherein the binder comprises a thermoplastic binder, a thermosetting binder, or a combination thereof.

6. The paint of claim 4, comprising an additive selected from the group consisting of an accelerator, an adhesion promoter, an antifoamer, anti-insect additive, an antioxidant, an anti-skinning agent, a buffer, a catalyst, a coalescing agent, a corrosion inhibitor, a crosslinking agent, a defoamer, a dehydrator, a dispersant, a drier, electrical additive, an emulsifier, a filler, a flame/fire retardant, a flatting agent, a flow control agent, a gloss aid, a leveling agent, a marproofing agent, a preservative, a silicone additive, a slip agent, a surfactant, a light stabilizer, a rheological control agent, a wetting additive, and combinations thereof.

7. The paint of claim 6, wherein the additive comprises 0.001% to 20.0% by weight, of the paint.

8. The paint of claim 7, wherein the binder comprises a polymer resin, wherein the additive comprises a combination of additives, wherein the combination of additives comprises a crosslinking agent and at least two ultraviolet light stabilizers whereby at least one light stabilizer is a sterically hindered amine, and at least one light stabilizer is a UV absorber, said UV absorber present at a concentration in excess of 5% by weight.

9. The paint of claim 8, wherein said sterically hindered amine is bis(1,2,2,6,6,-pentamethyl-4-poperidinyl) ester or bis(2,2,6,6,-tetramethyl-1-isooctyloxy-4-piperidinyl) ester.