Devices, systems and methods for linearization of polymers
First Claim
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1. A system for sequencing a biopolymer, comprising:
- a nanochannel having a detection region for detecting said biopolymer or a portion thereof upon flow of said biopolymer through said detection region; and
a first entropic trapping component and a second entropic trapping component disposed in or adjacent to said nanochannel, wherein (i) said first entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said first entropic trapping component and towards a first side of said detection region, and (ii) said second entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said second entropic trapping component and towards a second side of said detection region, which first side is different from said second side,wherein said first entropic trapping component is in said nanochannel, and wherein said first entropic trapping component includes an increase in cross-section of said nanochannel followed by a decrease in cross-section of said nanochannel towards said detection region.
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Abstract
The present disclosure provides methods and structures for systems which can linearize and capture a nucleic acid molecule (e.g., DNA) for re-measurement of the nucleic acid molecule or other polymer prior to detection of the polymer. The structures may allow for quick exchange between different samples or other reagents.
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Citations
18 Claims
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1. A system for sequencing a biopolymer, comprising:
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a nanochannel having a detection region for detecting said biopolymer or a portion thereof upon flow of said biopolymer through said detection region; and a first entropic trapping component and a second entropic trapping component disposed in or adjacent to said nanochannel, wherein (i) said first entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said first entropic trapping component and towards a first side of said detection region, and (ii) said second entropic trapping component is operable to linearize said biopolymer upon flow of said biopolymer through said second entropic trapping component and towards a second side of said detection region, which first side is different from said second side, wherein said first entropic trapping component is in said nanochannel, and wherein said first entropic trapping component includes an increase in cross-section of said nanochannel followed by a decrease in cross-section of said nanochannel towards said detection region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification