×

Precision gene targeting to a particular locus in maize

  • US 10,415,046 B2
  • Filed: 10/31/2016
  • Issued: 09/17/2019
  • Est. Priority Date: 12/13/2012
  • Status: Active Grant
First Claim
Patent Images

1. A method for integrating one or more exogenous nucleic acid sequences into the genome of a maize cell having an E32 locus, said method comprising the steps of:

  • making a double-stranded cleavage in the E32 locus using a site specific zinc finger nuclease, wherein the site specific zinc finger nuclease comprises three or more zinc finger motifs selected from the group consisting of SEQ ID NO;

    43, SEQ ID NO;

    44, SEQ ID NO;

    45, SEQ ID NO;

    46, SEQ ID NO;

    47, SEQ ID NO;

    48, SEQ ID NO;

    49, SEQ ID NO;

    50, SEQ ID NO;

    51, and SEQ ID NO;

    52; and

    ligating a polynucleotide comprising one or more exogenous nucleic acid sequences into the cleavage using a ligase or a recombinase, wherein the polynucleotide is integrated into the cleavage.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×