Methods and apparatus for detecting molecular interactions using FET arrays
First Claim
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1. A method for expression profiling comprising:
- providing a sensor array device including an array of sensor elements, wherein each sensor element includes a chemical detection circuit comprising;
a chemically-sensitive field effect transistor having a body terminal, a first terminal and a second terminal, the body terminal coupled to a body biasing voltage source line and the first terminal coupled to a first readout signal line;
a first switch coupling the second terminal of the chemically-sensitive field effect transistor to a current source; and
a second switch coupling the second terminal of the chemically-sensitive field effect transistor to a second readout signal line;
introducing a first nucleic acid analyte to the sensor array device, wherein the first nucleic acid analyte is obtained from a first sample in a first state;
determining a first expression level of the first nucleic acid analyte;
introducing a second nucleic acid analyte to the sensor array device, wherein the second nucleic acid analyte is obtained from a second sample in a second state;
determining a second expression level of the second nucleic acid analyte; and
comparing the first expression level and the second expression level.
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Abstract
Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.
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6 Claims
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1. A method for expression profiling comprising:
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providing a sensor array device including an array of sensor elements, wherein each sensor element includes a chemical detection circuit comprising; a chemically-sensitive field effect transistor having a body terminal, a first terminal and a second terminal, the body terminal coupled to a body biasing voltage source line and the first terminal coupled to a first readout signal line; a first switch coupling the second terminal of the chemically-sensitive field effect transistor to a current source; and a second switch coupling the second terminal of the chemically-sensitive field effect transistor to a second readout signal line; introducing a first nucleic acid analyte to the sensor array device, wherein the first nucleic acid analyte is obtained from a first sample in a first state; determining a first expression level of the first nucleic acid analyte; introducing a second nucleic acid analyte to the sensor array device, wherein the second nucleic acid analyte is obtained from a second sample in a second state; determining a second expression level of the second nucleic acid analyte; and comparing the first expression level and the second expression level. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification