Methods for producing canola plants with clubroot resistance and compositions thereof
First Claim
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1. A method for producing a clubroot-resistant canola plant, said method comprising:
- a) crossing a first clubroot-resistant canola plant comprising at least one clubroot resistance Quantitative Trait Locus (QTL) linked to a polymorphic marker selected from the group consisting of SEQ ID NOs;
1 to 7 with a second canola plant to produce a population of canola plants;
b) detecting in said population of canola plants a marker linked within 10 cM to said polymorphic marker selected from the group consisting of SEQ ID NOs;
1 to 7; and
c) selecting a clubroot-resistant canola plant comprising said at least one clubroot resistance QTL from said population;
wherein said clubroot resistance QTL is linked to at least one polymorphic marker selected from the grouping consisting of;
a G nucleotide at position 61 of SEQ ID NO;
1;
a C nucleotide at position 61 of SEQ ID NO;
2;
an A nucleotide at position 61 of SEQ ID NO;
3;
a G nucleotide at position 61 of SEQ ID NO;
4;
a G nucleotide at position 150 of SEQ ID NO;
5;
a G nucleotide at position 61 of SEQ ID NO;
6; and
an A nucleotide at position 61 of SEQ ID NO;
7.
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Abstract
The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding canola plants having clubroot resistance using marker-assisted selection. The disclosure further provides germplasm resistant to various Plasmodiophora brassicae pathotypes including pathogtype 5x. The disclosure also provides markers associated with clubroot resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel clubroot resistant germplasm, e.g., spring canola varieties resistant to pathogtype 5x.
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20 Claims
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1. A method for producing a clubroot-resistant canola plant, said method comprising:
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a) crossing a first clubroot-resistant canola plant comprising at least one clubroot resistance Quantitative Trait Locus (QTL) linked to a polymorphic marker selected from the group consisting of SEQ ID NOs;
1 to 7 with a second canola plant to produce a population of canola plants;b) detecting in said population of canola plants a marker linked within 10 cM to said polymorphic marker selected from the group consisting of SEQ ID NOs;
1 to 7; andc) selecting a clubroot-resistant canola plant comprising said at least one clubroot resistance QTL from said population; wherein said clubroot resistance QTL is linked to at least one polymorphic marker selected from the grouping consisting of; a G nucleotide at position 61 of SEQ ID NO;
1;a C nucleotide at position 61 of SEQ ID NO;
2;an A nucleotide at position 61 of SEQ ID NO;
3;a G nucleotide at position 61 of SEQ ID NO;
4;a G nucleotide at position 150 of SEQ ID NO;
5;a G nucleotide at position 61 of SEQ ID NO;
6; andan A nucleotide at position 61 of SEQ ID NO;
7. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification