Nucleic acid based data storage

• US 10,417,457 B2
• Filed: 09/19/2017
• Issued: 09/17/2019
• Est. Priority Date: 09/21/2016
• Status: Active Grant

• Current Assignee: Twist Bioscience Corporation
• Original Assignee: Twist Bioscience Corporation
• Inventors: Peck, Bill James
• Primary Examiner(s): Holder, Bradley W
• Application Number:
  US15/709,274
• Publication Number:
  US 20180101487A1
• Time in Patent Office: 728 Days
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12N 15/102 Mutagenizing nucleic acids
• CPC Class Codes: C12N 15/1027 by DNA shuffling, e.g. RSR, STEP, RPR
• CPC Class Codes: C12N 15/1089 Design, preparation, screening or analysis of libraries using computer algorithms
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: G06F 13/161 with latency improvement
• CPC Class Codes: G06F 13/4234 being a memory bus
• CPC Class Codes: G06F 21/78 to assure secure storage of data address-based protection against unauthorised use of memory G06F12/14; record carriers for use with machines and with at least a part designed to carry digital markings G06K19/00
• CPC Class Codes: G06F 2212/1052 Security improvement
• CPC Class Codes: G06F 2212/402 Encrypted data

Abstract

Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.

Claims

1. A method for storing and accessing information, the method comprising:

a) converting at least one item of information in a form of at least one digital sequence to at least one nucleic acid sequence;

b) providing a structure comprising a surface;

c) synthesizing a plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence, wherein each polynucleotide extends from the surface;

d) storing the plurality of polynucleotides; and

e) selectively transferring the plurality of polynucleotides to a receiving unit, wherein selectively transferring comprises application of electrostatic force, and wherein the plurality of polynucleotides collectively encodes for a single nucleic acid sequence of the at least one nucleic acid sequence.

2. The method of claim 1, wherein selectively transferring the plurality of polynucleotides to the receiving unit further comprises a conducting member, and an applied voltage potential between the structure and the conducting member.

3. The method of claim 1, wherein selectively transferring the plurality of polynucleotides to the receiving unit further comprises a pressure release or pressure nozzle.

4. The method of claim 3, wherein synthesizing the plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence further comprises using the pressure nozzle.

5. The method of claim 3, wherein synthesizing the plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence further comprises flooding the polynucleotides through the pressure nozzle.

6. The method of claim 3, wherein synthesizing the plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence further comprises depositing nucleotides through the pressure nozzle.

7. The method of claim 1, further comprising:

sequencing the plurality of polynucleotides; and

assembling the at least one digital sequence.

8. The method of claim 7, wherein the at least one digital sequence assembled is 100% accurate compared to an initial at least one digital sequence.

9. A method for collection of information, the method comprising:

a) providing a structure comprising a surface, wherein the structure comprises;

a first plurality of polynucleotides having predetermined sequences collectively encoding for at least one nucleic acid sequence; and

a second plurality of polynucleotides having predetermined sequences collectively encoding for the at least one nucleic acid sequence, wherein the first plurality of polynucleotides and the second plurality of polynucleotides both extend from the surface and both encode for the same at least one nucleic acid sequence;

b) selectively separating a region of the structure comprising the first plurality of polynucleotides and removing the first plurality of polynucleotides from the surface, wherein removing the first plurality of polynucleotides from the surface comprises application of electrostatic force; and

c) sequencing and decrypting the at least one nucleic acid sequence to form at least one digital sequence encoding for an item of information.

10. The method of claim 9, wherein a region of the structure comprising the first plurality of polynucleotides comprises a cluster of channels or wells.

11. The method of claim 9, wherein the structure is a rigid structure.

12. The method of claim 9, wherein the structure is a flexible structure.

13. The method of claim 9, wherein a region of the structure comprising only a remaining portion of the structure lacking the first plurality of polynucleotides is spliced back together.

14. The method of claim 9, wherein removing the first plurality of polynucleotides from the surface further comprises a conducting member, and an applied voltage potential between the structure and the conducting member.

15. The method of claim 9, wherein removing the first plurality of polynucleotides from the surface further comprises a pressure release or pressure nozzle.

16. The method of claim 9, wherein each polynucleotide of the first plurality of nucleotides comprises at most 500 bases in length.

17. The method of claim 9, wherein each polynucleotide of the first plurality of nucleotides comprises at most 200 bases in length.

18. The method of claim 9, wherein each polynucleotide of the second plurality of nucleotides comprises at most 500 bases in length.

19. The method of claim 9, wherein each polynucleotide of the second plurality of nucleotides comprises at most 200 bases in length.

20. The method of claim 9, wherein an amount of the item of information is at least one gigabyte.

21. The method of claim 9, wherein an amount of the item of information is at least one terabyte.

22. The method of claim 9, wherein an amount of the item of information is at least one petabyte.