Transporter with a glucose sensor for determining viability of an organ or tissue

• US 10,420,337 B2
• Filed: 03/15/2013
• Issued: 09/24/2019
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Lifeline Scientific, Inc. (Shanghai Genext Medical Technology Co., Ltd.)
• Original Assignee: Lifeline Scientific, Inc. (Shanghai Genext Medical Technology Co., Ltd.)
• Inventors: Mayer, Steven L., Kravitz, David C., Mayer, Tracey H.
• Primary Examiner(s): Kosar, Aaron J
• Application Number:
  US13/838,066
• Publication Number:
  US 20140272923A1
• Time in Patent Office: 2,384 Days
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0242 Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
• CPC Class Codes: A01N 1/0247 for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
• CPC Class Codes: A01N 1/0273 Transport containers A01N1/0268 takes precedence

Abstract

An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue with a perfusate; and a sensor operatively connected to the perfusion circuit. The sensor includes a solid support to which is attached a recognition molecule that permits detection of the target agent. The recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents. The sensor also includes an enzyme that can catalyze the conversion of a substance to glucose. In the presence of the target agent the enzyme can convert the substance into glucose, which can then be detected and optionally measured by the sensor.

Claims

1. An apparatus configured to perfuse an organ or tissue, the organ or tissue is an organ selected from the group consisting of a liver, a kidney, a heart, a lung, and an intestine, the apparatus comprising:

a processor that is configured to output a determination and/or information relating to whether organs or tissues that otherwise would be discarded could be viable;

a perfusion circuit for perfusing the organ or tissue with a perfusate, the perfusion circuit comprising a purge flow path and a recirculating perfusate flow path, wherein the recirculating perfusate flow path is configured to provide perfusate to the organ or tissue and recirculate the perfusate; and

a plurality of sensors operatively connected to the one or more of the flow paths of the perfusion circuit, wherein the plurality of sensors includes at least;

a first sensor in the recirculating perfusate flow path, and

a second sensor in the purge flow path;

where each sensor of the plurality of sensors includes;

a solid support attached to a recognition molecule, the recognition molecule configured to specifically bind to a viability-indicating target agent in the perfusate;

a substance that can be enzymatically converted to glucose;

an enzyme that can catalyze conversion of the substance to glucose in the presence of the viability-indicating target agent; and

a glucose meter configured to detect glucose produced from the substance, wherein the substance is selected from the group consisting of sucrose, maltose, trehalose, starch, and cellulose, where;

if the substance comprises sucrose, the enzyme comprises an invertase that can convert the sucrose to glucose, a sucrase that can convert the sucrose to glucose, or a sucrase-isomaltase that can convert the sucrose to glucose,

if the substance comprises maltose, the enzyme comprises a maltase that can convert maltose into glucose, if the substance comprises trehalose, the enzyme comprises a trehalase that can convert trehalose into glucose,

if the substance comprises starch, the enzyme comprises an amylase that can convert starch into glucose, and

if the substance comprises cellulose, the enzyme comprises a cellulase that can convert cellulose into glucose;

the viability-indicating target agent is an indicator of viability of the organ or tissue, the viability-indicating target agent comprising one or more members selected from the group consisting of lactate dehydrogenase, alanine aminotransferase, β

-galactosidase, TNF-α

, aspartate aminotransferase, IL-8, interleukins, antioxidant biomarkers, anti-apoptotic biomarkers, tropic factor biomarkers, and liver enzymes; and

the processor is configured to make the determination of whether the organs or tissues that otherwise would be discarded could be viable based on an amount of glucose detected.

2. The apparatus according to claim 1, wherein the solid support comprises a bead or a membrane.

3. The apparatus according to claim 1, wherein the recognition molecule includes a nucleic acid molecule, a protein, a polymer, or an antibody that specifically binds to the viability-indicating target agent.

4. The apparatus according to claim 1, wherein each sensor of the plurality of sensors being configured to sense the presence of a viability-indicating target agent specific to that sensor.

5. The apparatus according to claim 4, wherein each sensor of the plurality of sensors is configured to detect a different viability-indicating target agent.

6. The apparatus according to claim 4, wherein each sensor of the plurality of sensors is configured to detect the same viability-indicating target agent.

7. The apparatus according to claim 1, wherein

the processor also is configured to output information regarding an amount of the glucose detected by the glucose meter of each sensor of the plurality of sensors to at least one of a display screen of the apparatus and an external device via a wireless communication.

8. The apparatus according to claim 1, wherein

the processor is configured to compare an amount of glucose detected by the glucose meter of each sensor of the plurality of sensors with a baseline glucose level and quantitatively determines an amount of the viability-indicating target agent present in the perfusate based on a difference between the baseline glucose level and the amount of glucose detected.

9. The apparatus according to claim 1, wherein the viability-indicating target agent is selected from the group consisting of interleukins, aspartate aminotransferase, and alanine aminotransferase.

10. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a kidney.

11. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a kidney and the viability-indicating target agent is an interleukin.

12. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a liver and the viability-indicating target agent is one or more member selected from the group consisting of aspartate aminotransferase and alanine aminotransferase.

13. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a liver and the viability-indicating target agent is aspartate aminotransferase.

14. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a liver and the viability-indicating target agent is alanine aminotransferase.

15. The apparatus according to claim 1, wherein the apparatus is configured to perfuse a kidney and the viability-indicating target agent is one or more member selected from the group consisting of interleukins, IL-8, β

-galactosidase, and TNF-α

.

16. An apparatus for perfusing an organ or tissue, the apparatus being configured to perfuse a kidney or kidney tissue, the apparatus comprising:

a processor that is configured to output a determination and/or information relating to whether kidneys or kidney tissues that otherwise would be discarded could be viable;

a perfusion circuit for perfusing the kidney or kidney tissue with a perfusate, the perfusion circuit comprising a purge flow path and a recirculating perfusate flow path, wherein the recirculating perfusate flow path is configured to provide perfusate to the kidney or kidney tissue and recirculate the perfusate; and

a plurality of sensors operatively connected to the one or more of the flow paths of the perfusion circuit;

wherein the plurality of sensors includes at least;

a first sensor in the recirculating perfusate flow path, and

a second sensor in the purge flow path;

where each sensor of the plurality of sensors includes;

a solid support attached to a recognition molecule, the recognition molecule configured to specifically bind to a viability-indicating target agent in the perfusate, wherein the viability-indicating target agent is Kidney Injury Molecule-1 (KIM-1);

a substance that can be enzymatically converted to glucose;

an enzyme that can catalyze conversion of the substance to glucose in the presence of the viability-indicating target agent; and

a glucose meter configured to detect glucose produced from the substance, wherein the substance is selected from the group consisting of sucrose, maltose, trehalose, starch, and cellulose, where

if the substance comprises sucrose, the enzyme comprises an invertase that can convert the sucrose to glucose, a sucrase that can convert the sucrose to glucose, or a sucrase-isomaltase that can convert the sucrose to glucose,

if the substance comprises maltose, the enzyme comprises a maltase that can convert maltose into glucose,

if the substance comprises trehalose, the enzyme comprises a trehalase that can convert trehalose into glucose,

if the substance comprises starch, the enzyme comprises an amylase that can convert starch into glucose, and

if the substance comprises cellulose, the enzyme comprises a cellulase that can convert cellulose into glucose;

the viability-indicating target agent is an indicator of viability of the kidney or kidney tissue, and

the processor is configured to make the determination of whether the organs or tissues that otherwise would be discarded could be viable based on an amount of glucose detected.

17. The apparatus according to claim 16, wherein the enzyme is attached to a KIM-1 analogue molecule that competes less strongly than KIM-1 for binding to the recognition molecule.

18. The apparatus according to claim 16, wherein the enzyme is attached to a molecule that binds to KIM-1 that is bound to the recognition molecule.

19. An apparatus for perfusing an organ or tissue, the apparatus being configured to perfuse a liver or liver tissue, the apparatus comprising:

a processor that is configured to output a determination and/or information relating to whether liver or liver tissues that otherwise would be discarded could be viable;

a perfusion circuit for perfusing the liver or liver tissue with a perfusate, the perfusion circuit comprising a purge flow path and a recirculating perfusate flow path, wherein the recirculating perfusate flow path is configured to provide perfusate to the liver or liver tissue and recirculate the perfusate; and

a plurality of sensors operatively connected to the one or more of the flow paths of the perfusion circuit;

wherein the plurality of sensors includes at least;

a first sensor in the recirculating perfusate flow path, and

a second sensor in the purge flow path;

where each sensor of the plurality of sensors includes;

a solid support attached to a recognition molecule, the recognition molecule configured to specifically bind to a viability-indicating target agent in the perfusate, wherein the viability-indicating target agent is one or more member select from the group consisting of liver enzymes, lactate dehydrogenase and alanine aminotransferase;

a substance that can be enzymatically converted to glucose;

an enzyme that can catalyze conversion of the substance to glucose in the presence of the viability-indicating target agent; and

a glucose meter configured to detect glucose produced from the substance, wherein the substance is selected from the group consisting of sucrose, maltose, trehalose, starch, and cellulose, where

if the substance comprises sucrose, the enzyme comprises an invertase that can convert the sucrose to glucose, a sucrase that can convert the sucrose to glucose, or a sucrase-isomaltase that can convert the sucrose to glucose,

if the substance comprises maltose, the enzyme comprises a maltase that can convert maltose into glucose,

if the substance comprises trehalose, the enzyme comprises a trehalase that can convert trehalose into glucose,

if the substance comprises starch, the enzyme comprises an amylase that can convert starch into glucose, and

if the substance comprises cellulose, the enzyme comprises a cellulase that can convert cellulose into glucose;

the viability-indicating target agent is an indicator of viability of the liver or liver tissue; and

the processor is configured to make the determination of whether the organs or tissues that otherwise would be discarded could be viable based on an amount of glucose detected.