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Method and apparatus for the discretization and manipulation of sample volumes

  • US 10,421,070 B2
  • Filed: 09/29/2015
  • Issued: 09/24/2019
  • Est. Priority Date: 08/15/2008
  • Status: Active Grant
First Claim
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1. An apparatus comprising:

  • a flow channel having a flow axis, the flow channel being in selective fluid communication with a source of a continuous fluid comprising an aqueous solution;

    a first fluidic harbor offset from the flow axis and fluidically coupled to the flow channel at a first location of the flow channel and at a second location of the flow channel;

    a second fluidic harbor offset from the flow axis and fluidicially coupled to the flow channel at a first plurality of locations upstream of the first location along the flow channel;

    a third fluidic harbor offset from the flow axis and fluidically coupled to the flow channel at a second plurality of locations downstream of the second location along the flow channel; and

    a primer solution immiscible with the aqueous solution, the primer solution disposed within at least two of the fluidic harbors;

    wherein the first location is upstream of the second location along the flow channel.

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