Automated cell processing methods, modules, instruments, and systems
First Claim
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1. An automated stand-alone multi-module cell editing instrument for performing recursive nuclease editing comprising:
- a receptacle configured to receive cells;
one or more receptacles configured to receive nucleic acids;
a growth module for growing cells for transformation;
a filtration module to concentrate the cells and render the cells electrocompetent;
a transformation module configured to introduce the nucleic acids into the cells;
a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells;
a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and
an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the filtration module, from the filtration module to the transformation module, from the transformation module to the nuclease-directed editing module, and from the nuclease-directed editing module to the growth module, without user intervention.
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Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
106 Citations
20 Claims
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1. An automated stand-alone multi-module cell editing instrument for performing recursive nuclease editing comprising:
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a receptacle configured to receive cells; one or more receptacles configured to receive nucleic acids; a growth module for growing cells for transformation; a filtration module to concentrate the cells and render the cells electrocompetent; a transformation module configured to introduce the nucleic acids into the cells; a nuclease-directed editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells; a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the filtration module, from the filtration module to the transformation module, from the transformation module to the nuclease-directed editing module, and from the nuclease-directed editing module to the growth module, without user intervention. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. An automated stand-alone multi-module cell editing instrument for performing recursive nuclease editing comprising:
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a receptacle configured to receive cells; a receptacle configured to receive nucleic acids; a growth module comprising at least one rotating growth vial configured to grow the cells; a filtration module configured to concentrate the cells and render the cells electrocompetent; a transformation module comprising an electroporator to introduce the nucleic acids into the cells; a nuclease-directed editing module configured to select for transformed cells and to allow the nucleic acids to edit nucleic acids in the cells to produce edited cells; a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a script; and an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the filtration module, from the filtration module to the transformation module, from the nucleic acid receptacle to the transformation module, from the transformation module to the nuclease-directed editing module, and from the nuclease-directed editing module to the growth module, all without user intervention. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification