Cell culturing device

• US 10,428,300 B2
• Filed: 10/15/2013
• Issued: 10/01/2019
• Est. Priority Date: 10/15/2013
• Status: Active Grant

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Kiyama, Masaharu, Zhou, Guangbin, Nozaki, Takayuki
• Primary Examiner(s): Bowers, Nathan A
• Application Number:
  US15/027,485
• Publication Number:
  US 20160244711A1
• Time in Patent Office: 2,177 Days
• CPC Class Codes: C12M 29/26 Conditioning fluids entering or exiting the reaction vessel
• CPC Class Codes: C12M 41/34 of gas

Abstract

Maintaining pressure inside a culturing vessel is difficult when minimizing the size of a liquid supply path that supplies liquid and gas to a cell culturing vessel that performs two-level culturing (co-culturing). This cell culturing device includes: culturing vessels; a flow path having at least first supply ports capable of supplying liquid or gas to the culturing vessels and first discharge ports that discharge the gas from the culturing vessels, said flow path discharging gas from the first discharge ports to the atmosphere; and a filter in the flow path. A trap bottle is provided between the first discharge ports and the filter upon the flow path to collect moisture from the discharged gas. Thus, a liquid phase and a gas phase are separated in the trap bottle; moisture is prevented from reaching the filter; filter clogging is prevented; and the internal pressure of the culturing vessels can be maintained.

Claims

1. An automatic cell culture system comprising:

a culture vessel comprising a first vessel and a second vessel, the first vessel being positioned within the second vessel and having a culture surface formed of a permeable membrane;

wherein the first vessel comprises a first port configured to supply a liquid into the first vessel and discharge a gas from the first vessel, and a second port configured to discharge a liquid from the first vessel;

wherein the second vessel comprises a third port configured to supply a liquid into the second vessel and discharge a gas from the second vessel, and a fourth port configured to discharge a liquid from the second vessel;

the culture vessel further comprising;

at least one flow channel for discharging the gas discharged from the first port and the third port to outside air at atmospheric pressure;

at least one filter provided in the at least one flow channel; and

at least one collecting unit disposed between the first port and the third port, and the at least one filter in the at least one flow channel, the at least one collecting unit collecting moisture from the discharged gas.

2. The automatic cell culture system according to claim 1,

wherein the at least one collecting unit comprises a trap bottle.

3. The automatic cell culture system according to claim 2,

further comprising a refrigerator unit that holds the trap bottle at a low temperature.

4. The automatic cell culture system according to claim 2,

wherein the trap bottle is used once.

5. The automatic cell culture system according to claim 2,

wherein the trap bottle is transparent.

6. The automatic cell culture system according to claim 2,

wherein the trap bottle comprises a lid, through which an atmosphere side opening and a culture vessel side opening are penetrated and

an opening end of the atmosphere side opening is positioned higher than an opening end of the culture vessel side opening.

7. The automatic cell culture system according to claim 2,

further comprising a liquid bottle configured to hold the liquid supplied to the first supply port,

wherein the trap bottle stores the liquid supplied from the liquid bottle, not via the culture vessel.

8. The automatic cell culture system according to claim 1,

wherein the at least one collecting unit comprises a trap bottle.

9. The automatic cell culture system according to claim 8,

wherein the trap bottle holds an adsorbent in an inside thereof.

10. The automatic cell culture system according to claim 8,

further comprising a refrigerator unit configured to hold the trap bottle at a low temperature.

11. The automatic cell culture system according to claim 8,

wherein the trap bottle is used once.

12. The automatic cell culture system according to claim 8,

wherein the trap bottle is transparent.

13. The automatic cell culture system according to claim 8,

wherein the trap bottle comprises a lid through which an atmosphere side opening and a culture vessel side opening are penetrated and

an opening end of the atmosphere side opening is positioned higher than an opening end of the culture vessel side opening.

14. The automatic cell culture system according to claim 8,

further comprising a liquid bottle configured to hold the liquid supplied to the first supply port,

wherein the trap bottle stores the liquid supplied from the liquid bottle, not via the culture vessel.