Methods for heating microscope slides carrying specimens

• US 10,429,280 B2
• Filed: 06/15/2018
• Issued: 10/01/2019
• Est. Priority Date: 11/12/2008
• Status: Active Grant

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Beer, Jonathan Peter
• Primary Examiner(s): Paschall, Mark H
• Application Number:
  US16/010,357
• Publication Number:
  US 20180321116A1
• Time in Patent Office: 473 Days
• Field of Search: 435404-406, 219494, 219385, 219486, 219506, 392382
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/00168 Manufacturing or preparing test elements
• CPC Class Codes: G01N 2035/00346 Heating or cooling arrangements
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J

Abstract

A slide processing apparatus controls the temperature and orientation of a microscope slide carrying one or more specimens. The apparatus heats the specimen-bearing microscope slide while the slide is oriented to both facilitate adhesion between the specimens and the slide and control movement of the specimens relative to the microscope slide. A slide dryer of the apparatus conductively heats the specimens using a conductive slide heater that physically engages the microscope slide.

Claims

1. A method for processing microscope slides carrying specimens, the method comprising:

robotically delivering the microscope slides to a drying station, the microscope slides carrying transfer liquid and the specimens, which include tissue sections;

moving the microscope slides within the drying station such that the microscopic slides are positioned at an angle of inclination to promote draining of the transfer liquid from the microscope slides;

heating the microscope slides held at the angle of inclination such that a temperature along each of the microscope slides is higher than about 50 degrees Celsius to at least partially dry specimens carried by the microscope slides;

after at least partially drying the specimens,

robotically delivering the microscope slides from the drying station to a staining station;

delivering stains onto the microscope slides, carrying the at least partially dried specimens, using the staining station;

robotically transporting the microscope slides from the staining station to a cover-slipping station; and

robotically placing coverslips on the microscope slides using the cover-slipping station.

2. The method of claim 1, further comprising transporting the microscope slides between the drying station, the staining station, and the cover-slipping station using a transport device having a slide transporter that holds the microscope slides.

3. The method of claim 1, wherein robotically delivering the microscope slides from the drying station to the staining station includes carrying the microscope slides away from the drying station toward the staining station using a slide transporter device.

4. The method of claim 1, wherein the angle of inclination is greater than about 75 degrees.

5. The method of claim 1, further comprising operating a slide dryer of the drying station to heat the specimens to a temperature higher than 50 degrees Celsius to melt embedding material.

6. The method of claim 1, further comprising heating the microscope slides using a slide dryer in the drying station while the microscope slides are held in a predetermined spaced apart arrangement.

7. The method of claim 1, further comprising robotically transporting the microscope slides into the slide dryer using a slide handler which positions the microscope slides at the angle of inclination.

8. The method of claim 1, further comprising holding the microscope slides in a slide tray.

9. The method of claim 1, further comprising vertically moving the microscope slides to sequentially position a slide transporter, which carries the microscope slides, adjacent to the drying station, the staining station, and the cover-slipping station.

10. The method of claim 1, further comprising heating a heating element of the slide dryer to a temperature equal to or greater than about 50 degrees Celsius to heat the microscope slides.

11. The method of claim 1, further comprising:

allowing liquid to drain from surfaces of the microscope slides due to gravity while the microscope slides are held at the angle of inclination; and

heating, via conduction or convection, the specimens.

12. The method of claim 1, wherein at least one of the microscope slides carries a first specimen and the transfer liquid when the at least one microscope slide is delivered to the drying station, the method further comprises:

draining the transfer liquid from between at least a portion of the first specimen and the at least one microscope slide prior to removing the at least one microscope slide from the drying station.

13. The method of claim 1, wherein the drying station conductively heats the microscope slides held at the angle of inclination.

14. The method of claim 1, wherein the drying station is a convection oven.

15. A method for processing a microscope slide carrying a specimen, the method comprising:

robotically delivering the microscope slide, which is carrying the specimen that is wet, to a drying station with a slide heater configured to heat the specimen to a temperature higher than 50 degrees Celsius;

heating the specimen while the microscope slide is within the drying station and held at a substantially vertical orientation;

robotically delivering the microscope slide to a processing station after the drying station has dried at least a portion of the specimen to promote adhesion of the specimen to the microscope slide; and

dispensing one or more fluids using the processing station such that the one or more fluids contact the specimen carried by the microscope slide.

16. The method of claim 15, wherein after the drying station receives the microscope slide, a slide handler of the drying station moves the microscope slide to the substantially vertical orientation.

17. The method of claim 15, wherein the microscope slide at the substantially vertical orientation is held at an angle of inclination greater than about 75 degrees.

18. The method of claim 15, further comprising operating a slide dryer of the drying station to heat the specimen to a temperature higher than 50 degrees Celsius.

19. The method of claim 15, further comprising robotically rotating the microscope slide to position the microscope slide at the substantially vertical orientation.

20. The method of claim 15, further comprising heating the specimen via convection using the drying station.

21. A method for processing specimen-bearing microscope slides, the method comprising:

robotically delivering microscope slides to a drying station;

moving the microscope slides using the drying station to position the microscope slides at an inclined position;

heating the microscope slides held at the inclined position such that a temperature along the microscope slides is higher than about 50 degrees Celsius;

after at least partially drying specimens carried by the microscope slides, robotically delivering the microscope slides to a staining station; and

starting a staining protocol that includes robotically delivering stains onto the microscope slides using the staining station to stain the at least partially dried specimens.

22. The method of claim 21, further comprising transporting the microscope slides between the drying station and the staining station using a transport device while a slide tray holds the microscope slides.

23. The method of claim 21, further comprising holding the microscope slides in a slide tray to transport the microscope slides during processing.

24. The method of claim 21, wherein one or more of the microscope slides at the inclined position define an angle of inclination greater than about 75 degrees.

25. A method for processing specimen-bearing microscope slides, the method comprising:

robotically positioning the microscope slides at an inclined position;

heating the microscope slides held at the inclined position such that a temperature along the microscope slides is higher than about 50 degrees Celsius;

after heating the microscope slides to at least partially dry specimens on the microscope slides, robotically moving the microscope slides to staining positions; and

robotically delivering stains onto the microscope slides to stain the at least partially dried specimens.

26. The method of claim 25, further comprising robotically transporting the microscope slides between a drying station, a staining station, and a cover-slipping station.

27. The method of claim 25, wherein the microscope slides are held at the inclined position while reagent drains off of the microscope slides.

28. The method of claim 25, wherein the microscope slides are heated via convection or conduction.