System and method for desorbing and detecting an analyte sorbed on a solid phase microextraction device
First Claim
1. A system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device, the system comprising:
- a desorption chamber configured to be open to atmospheric pressure during operation and sized to accept the SPME device while defining a void volume of less than about 50 μ
L;
a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector;
a solvent source in fluid connection with the flow injector; and
a fluid switch that;
(a) in a desorption position, is configured to allow the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and(b) in an detecting position, is configured to isolate the solvent source from the flow injector by turning off the solvent flow while maintaining the fluid connection between the flow injector and the desorption chamber so as to transfer desorption solution in the desorption chamber through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid.
1 Assignment
0 Petitions
Accused Products
Abstract
Disclosed herein is a system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device. The system includes a desorption chamber sized to accept the SPME device while defining a void volume of less than about 50 μL; a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; and a fluid switch that: in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and in an detecting position, turns off the solvent source while maintaining the fluid connection between the flow injector and the desorption chamber, transferring the desorption solution through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid.
-
Citations
26 Claims
-
1. A system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device, the system comprising:
-
a desorption chamber configured to be open to atmospheric pressure during operation and sized to accept the SPME device while defining a void volume of less than about 50 μ
L;a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; and a fluid switch that; (a) in a desorption position, is configured to allow the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and (b) in an detecting position, is configured to isolate the solvent source from the flow injector by turning off the solvent flow while maintaining the fluid connection between the flow injector and the desorption chamber so as to transfer desorption solution in the desorption chamber through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A system for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device, the system comprising:
-
a desorption chamber sized to accept the SPME device while defining a void volume of less than about 50 μ
L;a flow injector in fluid connection with the desorption chamber, the desorption chamber and the flow injector being fluidly connected by at least a flow-insulating fluid connector; a solvent source in fluid connection with the flow injector; a 3-way junction fluidly connecting (1) the desorption chamber, (2) the solvent source, and (3) the flow injector; and a fluid switch that; (a) in a desorption position, allows the solvent to be sprayed from the flow injector while flow-insulating any desorption solution in the desorption chamber, and (b) in an detecting position, isolates the solvent source from the flow injector by turning off the solvent flow while maintaining the fluid connection between the flow injector and the desorption chamber so as to transfer desorption solution in the desorption chamber through the flow-insulating fluid connector to the flow injector as a substantially undiluted plug of liquid; wherein, during operation, the desorption chamber is configured to be open to atmospheric pressure and the flow injector is at a reduced pressure in comparison to the solvent source and the desorption chamber; wherein, during operation, the desorption chamber is arranged to reduce or prevent fluid from dripping out of the desorption chamber; and wherein the fluid switch is configured to change the relative flows of (a) fluid from the solvent source to (b) fluid drawn from the flow injector. - View Dependent Claims (12, 13, 14, 15)
-
-
16. A method for desorbing and detecting an analyte sorbed on a solid phase microextraction (SPME) device, the method comprising:
-
desorbing at least some of the analyte from the SPME device into a desorption solution in a desorption chamber that is in fluid connection with a flow injector, wherein the desorption solution in the desorption chamber is substantially not flowing to the flow injector; nebulizing a solvent from the flow injector while the analyte is desorbing into the desorption solution in the desorption chamber, wherein the solvent is nebulized from the flow injector at a rate sufficient to fluidly isolate the desorption solution in the desorption chamber from the solvent being nebulized; flushing substantially all of the desorption solution in the desorption chamber to the flow injector as a substantially undiluted plug of liquid; and spraying the desorption solution through the flow injector into a detection device. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
-
Specification