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Process control for increased robustness of genetic assays

  • US 10,429,399 B2
  • Filed: 09/18/2015
  • Issued: 10/01/2019
  • Est. Priority Date: 09/24/2014
  • Status: Active Grant
First Claim
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1. A method for processing a biological sample, the method comprising:

  • obtaining a sample comprising at least one cell biopsied from an embryo in a solution of 2-3 μ

    l of liquid;

    contacting the sample with an indicator dye comprising cresol red that provides a visual indication that the sample is frozen and within a specific, pre-defined pH range;

    exposing the sample to a freezing temperature;

    determining based on the visual indication that the sample is frozen; and

    performing a preimplantation genetic screening procedure comprising;

    using a camera controlled by computer software to evaluate a color of the sample;

    confirming with the computer software that the color indicates that the sample remains frozen;

    contacting the sample with an alkaline lysis solution followed by contacting the sample with an acid to achieve a neutral pH;

    using the camera and the computer software to evaluate a color of the sample and confirm that the sample has a neutral pH; and

    analyzing a karyotype of the at least one biopsied cell in the sample.

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