Process control for increased robustness of genetic assays
First Claim
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1. A method for processing a biological sample, the method comprising:
- obtaining a sample comprising at least one cell biopsied from an embryo in a solution of 2-3 μ
l of liquid;
contacting the sample with an indicator dye comprising cresol red that provides a visual indication that the sample is frozen and within a specific, pre-defined pH range;
exposing the sample to a freezing temperature;
determining based on the visual indication that the sample is frozen; and
performing a preimplantation genetic screening procedure comprising;
using a camera controlled by computer software to evaluate a color of the sample;
confirming with the computer software that the color indicates that the sample remains frozen;
contacting the sample with an alkaline lysis solution followed by contacting the sample with an acid to achieve a neutral pH;
using the camera and the computer software to evaluate a color of the sample and confirm that the sample has a neutral pH; and
analyzing a karyotype of the at least one biopsied cell in the sample.
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Abstract
The present disclosure describes a method of processing a biological sample. The method includes contacting the sample with a pH indicator that changes color in response to changes in pH. The presence of a pH indicator such as cresol red provides the benefit of improved visualization of the sample, verification that the sample is properly frozen and thawed, and increased precision in pH adjustments during the assay, among other benefits.
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Citations
7 Claims
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1. A method for processing a biological sample, the method comprising:
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obtaining a sample comprising at least one cell biopsied from an embryo in a solution of 2-3 μ
l of liquid;contacting the sample with an indicator dye comprising cresol red that provides a visual indication that the sample is frozen and within a specific, pre-defined pH range; exposing the sample to a freezing temperature; determining based on the visual indication that the sample is frozen; and performing a preimplantation genetic screening procedure comprising; using a camera controlled by computer software to evaluate a color of the sample; confirming with the computer software that the color indicates that the sample remains frozen; contacting the sample with an alkaline lysis solution followed by contacting the sample with an acid to achieve a neutral pH; using the camera and the computer software to evaluate a color of the sample and confirm that the sample has a neutral pH; and analyzing a karyotype of the at least one biopsied cell in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification