Methods and compositions for plant pest control
First Claim
Patent Images
1. A method for producing a soybean plant exhibiting an improvement in nematode disease resistance comprising topically applying to the soybean plant surface a composition that comprises:
- a. at least one exogenous double stranded RNA (dsRNA) of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO;
21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector and wherein the dsRNA is not transcribed from DNA integrated into a chromosome of the plant; and
b. a transfer agent that conditions the plant surface to permeation by the dsRNA into cells of the plant, wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene.
1 Assignment
0 Petitions
Accused Products
Abstract
Provided are methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. Also provided are combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants.
-
Citations
17 Claims
-
1. A method for producing a soybean plant exhibiting an improvement in nematode disease resistance comprising topically applying to the soybean plant surface a composition that comprises:
-
a. at least one exogenous double stranded RNA (dsRNA) of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO;
21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector and wherein the dsRNA is not transcribed from DNA integrated into a chromosome of the plant; andb. a transfer agent that conditions the plant surface to permeation by the dsRNA into cells of the plant, wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 15)
-
-
8. A composition comprising a dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO:
- 21, wherein said polynucleotide is not operably linked to a promoter or to a viral vector; and
a transfer agent that conditions a plant surface to permeation by the dsRNA into cells of the plant. - View Dependent Claims (9, 10, 11, 16)
- 21, wherein said polynucleotide is not operably linked to a promoter or to a viral vector; and
-
12. A soybean plant comprising an exogenous dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO:
- 21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, is not found in a non-transgenic soybean plant; and
wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of a endogenous DND1 gene. - View Dependent Claims (13, 14, 17)
- 21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, is not found in a non-transgenic soybean plant; and
Specification