Methods and compositions for plant pest control

• US 10,435,701 B2
• Filed: 08/21/2017
• Issued: 10/08/2019
• Est. Priority Date: 03/14/2013
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Crawford, Michael J., Gasper, Michelle L., Li, Xiangqian, Shortt, Barry J., Williams, Deryck Jeremy
• Primary Examiner(s): Ibrahim, Medina A
• Assistant Examiner(s): Zhong, Wayne
• Application Number:
  US15/682,205
• Publication Number:
  US 20180037899A1
• Time in Patent Office: 778 Days
• Field of Search: None
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8282 for fungal resistance
• CPC Class Codes: C12N 15/8285 for nematode resistance
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

Provided are methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. Also provided are combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants.

Claims

1. A method for producing a soybean plant exhibiting an improvement in nematode disease resistance comprising topically applying to the soybean plant surface a composition that comprises:

a. at least one exogenous double stranded RNA (dsRNA) of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO;

21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector and wherein the dsRNA is not transcribed from DNA integrated into a chromosome of the plant; and

b. a transfer agent that conditions the plant surface to permeation by the dsRNA into cells of the plant, wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene.

2. The method of claim 1, wherein said transfer agent comprises a surfactant, lipid, or combination thereof.

3. The method of claim 1, wherein said dsRNA is selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.

4. The method of claim 1, wherein said composition comprises any combination of two or more of said dsRNA molecules.

5. The method of claim 1, wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.

6. The method of claim 1, wherein said transfer agent comprises an organosilicone preparation.

7. A soybean plant obtained by the method of claim 1, said soybean plant comprising an exogenous dsRNA of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO:

21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, and is not found in a non-transgenic plant; and

wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene.

15. The method of claim 1, wherein said dsRNA of 25 to about 95 nucleotides in length comprises a sequence selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.

8. A composition comprising a dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO:

21, wherein said polynucleotide is not operably linked to a promoter or to a viral vector; and

a transfer agent that conditions a plant surface to permeation by the dsRNA into cells of the plant.

9. The composition of claim 8, wherein said dsRNA molecule is selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.

10. The composition of claim 8, wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.

11. The composition of claim 8, wherein said transfer agent is an organosilicone preparation.

16. The composition of claim 8, wherein said dsRNA molecule of 25 to about 95 nucleotides in length comprises a sequence selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.

12. A soybean plant comprising an exogenous dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO:

21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, is not found in a non-transgenic soybean plant; and

wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of a endogenous DND1 gene.

13. The soybean plant of claim 12, wherein said soybean plant further comprises an organosilicone compound or a component thereof.

14. The soybean plant of claim 12, wherein said dsRNA is selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.

17. The soybean plant of claim 12, wherein said dsRNA molecule of 25 to about 95 nucleotides in length comprise a sequence selected from the group consisting of SEQ ID NO:

38, 39, 43, and 45.