Viscometer and methods for using the same

• US 10,436,694 B2
• Filed: 10/11/2016
• Issued: 10/08/2019
• Est. Priority Date: 04/11/2014
• Status: Active Grant

• Current Assignee: RheoSense, Inc.
• Original Assignee: RheoSense, Inc.
• Inventors: Baek, Seong-Gi
• Primary Examiner(s): Huang, David Z
• Application Number:
  US15/290,936
• Publication Number:
  US 20170030818A1
• Time in Patent Office: 1,092 Days
• CPC Class Codes: G01N 11/02 by measuring flow of the material
• CPC Class Codes: G01N 11/08 by measuring pressure required to produce a known flow
• CPC Class Codes: G01N 2011/002 Controlling sample temperature; Thermal cycling during measurement
• CPC Class Codes: G01N 2011/0026 Investigating specific flow properties of non-Newtonian fluids
• CPC Class Codes: G01N 25/04 of melting point; of freezing point; of softening point
• CPC Class Codes: G01N 33/487 of liquid biological material

Abstract

A viscometer includes a viscosity sensor with a liquid flow channel and at least two pressure sensors positioned along the liquid flow channel and configured to measure a pressure drop of a liquid flowing through the liquid flow channel, and a dispensing mechanism configured to cause dispensing of a liquid from the syringe to the viscosity sensor at a known flow rate. The dispensing mechanism and the viscosity sensor are configured to couple with a syringe configured to contain a liquid. The viscometer further includes an electronic controller configured to control operations of the dispensing mechanism and receive and process data from the viscosity sensor. The viscometer includes a sample loading interface, included in the syringe, through which the viscometer is configured to receive the liquid. The sample loading interface includes a selection valve coupled with, and located between, the viscosity sensor and the syringe.

Claims

1. A viscometer, comprising:

a dispensing mechanism configured to cause dispensing of a liquid at a known flow rate from a first syringe to a viscosity sensor with a liquid flow channel and at least two pressure sensors positioned along the liquid flow channel and configured to measure a pressure drop of the liquid flowing through the liquid flow channel, including a first pressure sensor positioned to measure a pressure at a first location of the liquid flow channel and a second pressure sensor positioned to measure a pressure at a second location of the liquid flow channel, wherein the dispensing mechanism is configured to couple with the first syringe, and the first syringe is coupled with the viscosity sensor;

an electronic controller configured to control operations of the dispensing mechanism and receive and process data from the viscosity sensor; and

a sample loading interface through which the viscometer is configured to receive the liquid; and

an autosampler configured to move an injector syringe between a location for aspirating a test liquid into the injector syringe and a location for coupling the injector syringe with the sample loading interface to load the test liquid into the first syringe.

2. The viscometer of claim 1, further comprising a selection valve coupled with the viscosity sensor and the first syringe and located between the viscosity sensor and the first syringe.

3. The viscometer of claim 1, wherein a barrel of the first syringe includes a side port through which the barrel is coupled with the sample loading interface.

4. The viscometer of claim 1, wherein the viscometer is configured to aspirate the test liquid into the injector syringe independent of user intervention.

5. The viscometer of claim 1, further comprising a viscosity sensor module that includes a plurality of viscosity sensors.

6. The viscometer of claim 1, wherein a plurality of liquid flow channels is defined in the viscosity sensor and at least two pressure sensors are positioned along each of two or more liquid flow channels of the plurality of liquid flow channels.

7. The viscometer of claim 1, further comprising a temperature control device, wherein the temperature control device is coupled with the electronic controller and the electronic controller is configured to control the temperature control device;

determine viscosity values of the liquid at a plurality of temperatures, the liquid including proteins of a first type; and

determine a melting temperature of the proteins of the first type from viscosity values of the liquid at the plurality of temperatures.

8. The viscometer of claim 7, wherein the electronic controller is configured to:

determine viscosity values of a second liquid at a plurality of temperatures, the second liquid including proteins of a second type; and

determine a melting temperature of the proteins of the second type from viscosity values of the second liquid at the plurality of temperatures.

9. The viscometer of claim 1, further comprising a mixer coupled with the electronic controller and configured to mix the liquid with a solvent to obtain a mixed solution, wherein a concentration of a solute in the mixed solution is distinct from a concentration of the solute in the liquid, wherein the electronic controller is configured to:

measure a viscosity of the mixed solution; and

initiate repeated mixing and measuring to obtain viscosity values of the mixed solution for a plurality of concentrations of the solute.

10. The viscometer of claim 1, including a sample injector, the sample injector being configured to dispense a continuous stream of liquids to the viscosity sensor, the continuous stream of liquids including two or more batches of test liquids, wherein any two adjacent batches of test liquids, of the two or more batches of test liquids, are separated by at least one inert liquid immiscible with the two adjacent batches of test liquids.

11. The viscometer of claim 1, further comprising a pump configured to dispense a cleaning solution through the liquid flow channel of the viscosity sensor.

12. The viscometer of claim 1, wherein the viscometer is configured to couple with a source of gas and provide the gas through the liquid flow channel.

13. The viscometer of claim 12, wherein the gas is regulated dry gas.

14. The viscometer of claim 13, wherein the viscometer is configured to determine an operation status of the viscosity sensor based on one or more measurements for the regulated dry gas.

15. The viscometer of claim 1, wherein the viscometer is configured to self-calibrate by using a preselected reference liquid.

16. The viscometer of claim 1, further comprising one or more of:

a pH meter, a density meter, and a conductivity meter.

17. A method for performing a viscosity measurement, the method comprising:

moving, using an autosampler, an injector syringe between a first location for aspirating a liquid into the injector syringe and a second location for coupling the injector syringe with a sample loading interface to load the liquid into a first syringe, wherein the second location is distinct from the first location;

coupling the injector syringe with a viscosity sensor of a viscometer through the first syringe, wherein the viscosity sensor is with a liquid flow channel and at least two pressure sensors positioned along the liquid flow channel and configured to measure a pressure drop of the liquid flowing through the liquid flow channel, including a first pressure sensor positioned to measure a pressure at a first location of the liquid flow channel and a second pressure sensor positioned to measure a pressure at a second location of the liquid flow channel;

receiving the liquid from the injector syringe into the first syringe through the sample loading interface;

dispensing, with a dispensing mechanism coupled with the first syringe and an electronic controller, the liquid into the liquid flow channel of the viscosity sensor at a known rate; and

measuring a viscosity of the liquid.

18. The method of claim 17, further comprising:

coupling the viscometer with a source of gas, wherein the gas is regulated dry gas;

providing the gas through the liquid flow channel;

performing a measurement with the viscosity sensor; and

determining whether the viscosity sensor is operating within predefined criteria based on the measurement performed with the viscosity sensor.

19. The method of claim 18, wherein performing the measurement with the viscosity sensor includes measuring pressures of the gas within the viscosity sensor.

20. The method of claim 19, wherein performing the measurement with the viscosity sensor includes converting the pressures of the gas to a flow rate of the gas.