Methods of making quinoa leachates and uses thereof

• US 10,441,594 B2
• Filed: 12/05/2014
• Issued: 10/15/2019
• Est. Priority Date: 12/06/2013
• Status: Active Grant

• Current Assignee: Rutgers University
• Original Assignee: Rutgers University
• Inventors: Raskin, Ilya, Graf, Brittany
• Primary Examiner(s): Meller, Michael V
• Application Number:
  US14/562,238
• Publication Number:
  US 20150157644A1
• Time in Patent Office: 1,775 Days
• Field of Search: 424725
• CPC Class Codes: A61K 2236/00 Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 36/21 Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth

Abstract

The present application is directed to materials and method for producing leachates from a plant of the Amaranthaceae family.

Claims

1. A method of producing a leachate comprising 20-hydroxyecdysone from an intact quinoa seed comprising:

contacting the intact quinoa seed with a leaching fluid to produce a leaching mixture comprising the 20-hydroxyecdysone, wherein the leaching fluid is selected from the group consisting of water, n-butanol, isopropanol, n-propanol, aqueous ethanol having a concentration less than 95% ethanol, methanol, ethyl acetate, nitromethane and formic acid, and wherein the contacting step is performed at a temperature of less than 80°

C.; and

separating the quinoa seed from the leaching mixture, thereby producing a leachate comprising 20-hydroxyecdysone from the quinoa seed, wherein the leachate comprises from about 100 μ

g to about 400 μ

g 20-hydroxyecdysone per gram of seed.

2. The method of claim 1, wherein the quinoa seed is a Chenopodium quinoa plant seed.

3. The method of claim 1, wherein the leaching fluid comprises 60-80% ethanol.

4. The method of claim 3, wherein the leaching fluid comprises 70% ethanol.

5. The method of claim 1, wherein the contacting step is performed at room temperature.

6. The method of claim 1, wherein the separating step is performed using a method selected from the group consisting of filtration, sedimentation, centrifugation, evaporation, reduced-pressure distillation, precipitation, lyophilization and adsorption.

7. The method of claim 1, further comprising drying the leachate.

8. The method of claim 7, wherein the drying is performed by using a method selected from the group consisting of air drying, spray drying, speed vacuum, rotoevaporation, and lyophilization.