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Methods and products for transfection

  • US 10,443,045 B2
  • Filed: 04/03/2019
  • Issued: 10/15/2019
  • Est. Priority Date: 12/05/2011
  • Status: Active Grant
First Claim
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1. A method for reprogramming a differentiated cell to a less differentiated state, comprising:

  • (a) providing a differentiated cell;

    (b) culturing the differentiated cell; and

    (c) transfecting the differentiated cell with one or more synthetic RNA molecules,wherein the one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors;

    wherein the transfecting results in the cell expressing the one or more reprogramming factors; and

    wherein step (c) is performed at least twice and the amount of one or more synthetic RNA molecules transfected in one or more later transfections is greater than the amount transfected in one or more earlier transfections to result in the cell being reprogrammed to a less differentiated state and occurs in the presence of a medium containing ingredients that support reprogramming of the differentiated cell to a less differentiated state.

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