DNA polymerases from the red sea brine pool

• US 10,443,048 B2
• Filed: 04/10/2015
• Issued: 10/15/2019
• Est. Priority Date: 04/11/2014
• Status: Active Grant

• Current Assignee: King Abdullah University of Science and Technology
• Original Assignee: King Abdullah University of Science and Technology
• Inventors: Hamdan, Samir M., Takahashi, Masateru
• Primary Examiner(s): Hutson, Richard G
• Application Number:
  US15/302,986
• Publication Number:
  US 20170029793A1
• Time in Patent Office: 1,649 Days
• Field of Search: None
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C12Y 207/07007 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.

Claims

1. A method for amplifying a polynucleotide comprising:

reacting a DNA template, a primer, dNTPs and a DNA polymerase having the amino acid sequence of SEQ ID NO;

3, wherein the primer is extended in a polymerase chain reaction (PCR) to synthesize a DNA primer extension product.

2. A kit for amplifying a polynucleotide comprising a DNA polymerase having the amino acid sequence of SEQ ID NO:

3, dNTPs and a reaction buffer.

3. An expression vector for heterologous expression of a DNA polymerase, comprising a nucleic acid sequence encoding a DNA polymerase having the amino acid sequence of SEQ ID NO:

3 and a heterologous promoter.

4. The expression vector of claim 3 comprising a promoter for expression in an E. coli host cell.

5. The expression vector of claim 3, wherein the expression vector is a plasmid.

6. The expression vector of claim 3 in a composition comprising additional polynucleotides comprising the polynucleotide sequence of SEQ ID NO:

5 and SEQ ID NO;

6.