Plants with engineered endogenous genes

• US 10,443,068 B2
• Filed: 01/31/2017
• Issued: 10/15/2019
• Est. Priority Date: 06/25/2010
• Status: Active Grant

• Current Assignee: Agrivida, Inc. (Nippon Soda Company Limited)
• Original Assignee: Agrivida, Inc. (Nippon Soda Company Limited)
• Inventors: Raab, R. Michael, Lanahan, Michael, Bonin, Christopher, Bougri, Oleg
• Primary Examiner(s): Rosen, Jason Deveau
• Application Number:
  US15/420,480
• Publication Number:
  US 20170137836A1
• Time in Patent Office: 987 Days
• Field of Search: None
• CPC Class Codes: A23K 10/10 obtained by microbiological or biochemical processes using chemicals or microorganisms for ensilaging of green fodder A23K30/15
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 10/38 from distillers' or brewers' waste
• CPC Class Codes: A23K 40/10 by agglomeration; by granulation, e.g. making powders
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/30 for swines
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 15/8246 Non-starch polysaccharides, e.g. cellulose, fructans, levans
• CPC Class Codes: C12N 9/1294 Phosphotransferases with paired acceptors (2.7.9)
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12Y 207/00 Transferases transferring phosphorus-containing groups (2.7)
• CPC Class Codes: C12Y 207/09004 Alpha-glucan, water dikinase (2.7.9.4)
• CPC Class Codes: Y02P 60/87 Re-use of by-products of food processing for fodder production

Abstract

Genetically engineered plants expressing altered Glucan Water Dikinase and having elevated levels of starch are provided. Methods of genetically engineering plants to express altered Glucan Water Dikinase, and genetic constructs are provided. Methods of breeding genetically engineered plants homozygous for a mutated gene encoding an altered Glucan Water Dikinase are described. Methods of agricultural processing and animal feed using the genetically engineered plants are also provided.

Claims

1. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a mutation in a target nucleic acid sequence included in at least one allele of a gene encoding wild type Glucan Water Dikinase, the altered Glucan Water Dikinase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:

45-73, 121-124, 126-127, and 147-162, and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.

2. The genetically engineered plant of claim 1, wherein the altered Glucan Water Dikinase has an activity that is reduced compared to wild type Glucan Water Dikinase activity.

3. The genetically engineered plant of claim 1, wherein the altered Glucan Water Dikinase is inactive.

4. A method of preparing animal feed comprising processing a genetically engineered plant of claim 1, wherein processing includes at least one of operation selected from the group consisting of harvesting, bailing, grinding, milling, chopping, size reducing, crushing, extracting a component from the feedstock, purifying a component or portion of the feedstock, and extracting or purifying starch, wherein the animal feed comprises the engineered nucleic acid encoding the altered Glucan Water Dikinase.

5. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a polynucleotide sequence selected from the group consisting of SEQ ID NOS:

12-40, 106-107, 114-120, 131-146, and 188, and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.

6. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a mutation in a target nucleic acid sequence included in at least one allele of a gene encoding wild type Glucan Water Dikinase, the altered Glucan Water Dikinase comprises an amino acid sequence of SEQ ID NO:

194 or 195 and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.