Plants with engineered endogenous genes
First Claim
1. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a mutation in a target nucleic acid sequence included in at least one allele of a gene encoding wild type Glucan Water Dikinase, the altered Glucan Water Dikinase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 45-73, 121-124, 126-127, and 147-162, and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.
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Accused Products
Abstract
Genetically engineered plants expressing altered Glucan Water Dikinase and having elevated levels of starch are provided. Methods of genetically engineering plants to express altered Glucan Water Dikinase, and genetic constructs are provided. Methods of breeding genetically engineered plants homozygous for a mutated gene encoding an altered Glucan Water Dikinase are described. Methods of agricultural processing and animal feed using the genetically engineered plants are also provided.
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Citations
6 Claims
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1. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a mutation in a target nucleic acid sequence included in at least one allele of a gene encoding wild type Glucan Water Dikinase, the altered Glucan Water Dikinase comprises an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 45-73, 121-124, 126-127, and 147-162, and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.
- View Dependent Claims (2, 3, 4)
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5. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a polynucleotide sequence selected from the group consisting of SEQ ID NOS:
- 12-40, 106-107, 114-120, 131-146, and 188, and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.
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6. A genetically engineered plant comprising an engineered nucleic acid encoding an altered Glucan Water Dikinase, wherein the engineered nucleic acid comprises a mutation in a target nucleic acid sequence included in at least one allele of a gene encoding wild type Glucan Water Dikinase, the altered Glucan Water Dikinase comprises an amino acid sequence of SEQ ID NO:
- 194 or 195 and the plant has an elevated level of starch in comparison to a plant of the same genetic background comprising a wild type Glucan Water Dikinase.
Specification