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Synthesis of long-chain polyunsaturated fatty acids by recombinant cell

  • US 10,443,079 B2
  • Filed: 07/05/2017
  • Issued: 10/15/2019
  • Est. Priority Date: 04/22/2004
  • Status: Active Grant
First Claim
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1. A process for increasing the levels of long chain polyunsaturated fatty acid(s) (LC-PUFA) in fish comprising:

  • a) obtaining feedstuff which comprises oil extracted from transgenic Brassica seeds which comprise eicosapentaenoic acid (EPA) and heterologous polynucleotides which encode enzymes that comprise;

    i) a Δ

    6 desaturase, a Δ

    6 elongase and a Δ

    5 desaturase;

    orii) a Δ

    5/Δ

    6 bifunctional desaturase and a Δ

    5/Δ

    6 bifunctional elongase;

    wherein the heterologous polynucleotides are operably linked to one or more promoters that are capable of directing expression of said polynucleotides in Brassica seed cells, wherein the enzymes encoded by said polynucleotides comprise at least one desaturase which is able to act on an acyl-CoA substrate; and

    b) administering the feedstuff to fish,thereby increasing the levels of LC-PUFA in the fish.

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