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Selective oxidation of 5-methylcytosine by TET-family proteins

  • US 10,443,091 B2
  • Filed: 06/27/2016
  • Issued: 10/15/2019
  • Est. Priority Date: 09/26/2008
  • Status: Active Grant
First Claim
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1. A method for detecting 5-hydroxymethylcytosine in a nucleic acid, the method comprising contacting the nucleic acid with an enzyme and utilizing a glucose donor substrate or a glucose-derivative donor substrate that traps covalent enzyme-DNA intermediates to detect 5-hydroxymethylcytosine residues, wherein the enzyme comprises an alpha-glucosyltransferase, a beta-glucosyltransferase, or a beta-glucosyl-alpha-glucosyl-transferase.

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