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Method of measuring insulin resistance with fatty acid combustion, and composition used herein

  • US 10,444,229 B2
  • Filed: 03/13/2014
  • Issued: 10/15/2019
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
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1. A method for measuring insulin resistance of a test subject, comprisingdetermining an area under a Δ

  • -labeled C(‰

    )-expired air collection time t curve, based on a glucose metabolism ability measurement method having steps (i) and (ii) below (hereinafter referred to as “

    AUCt[labeled C-glucose]”

    ),determining an area under a Δ

    -labeled C(‰

    )-expired air collection time t curve, based on a method having steps (a) and (b) below (hereinafter referred to as “

    AUCt[labeled C-fatty acid]”

    ),using a value obtained by dividing the AUCt[labeled C-glucose] by the AUCt[labeled C-fatty acid] (AUCt[labeled C-glucose]/AUCt[labeled C-fatty acid]) as an index for the sugar/fatty acid combustion ratio,determining a first index of the sugar/fatty acid combustion ratio under a fasting condition and a second index of the sugar/fatty acid combustion ratio under a feeding condition from the test subject, anddetermining a difference between the first index and the second index, wherein the test subject'"'"'s insulin resistance is measured,whereinthe method in which AUCt[labeled C-glucose] is determined has the steps of;

    (i) intravenously administering a composition to a test subject and collecting expired air, the composition comprising, as an active ingredient, glucose labeled with at least one isotope of carbon, so that the glucose is converted in the body into labeled carbon dioxide that is excreted in expired air; and

    (ii) determining the ratio of labeled CO2 amount to unlabeled CO2 amount contained in expired air or the ratio of labeled CO2 amount to total CO2 amount contained in expired air, andthe method in which AUCt[labeled C-fatty acid] is determined has the steps of;

    (a) intravenously administering a composition comprising a C12-38 fatty acid or a salt thereof labeled with at least one isotope of carbon to a test subject and collecting expired air, so that the C12-38 fatty acid or salt thereof is converted in the body into labeled carbon dioxide that is excreted in expired air; and

    (b) determining the ratio of labeled CO2 amount to unlabeled CO2 amount contained in expired air or the ratio of labeled CO2 amount to total CO2 amount contained in expired air.

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