Modulation of expression of acyltransferases to modify hydroxycinnamic acid content

• US 10,450,578 B2
• Filed: 07/17/2017
• Issued: 10/22/2019
• Est. Priority Date: 12/21/2012
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Bartley, Laura E., Ronald, Pamela, Scheller, Henrik Vibe
• Primary Examiner(s): Kallis, Russell
• Application Number:
  US15/652,115
• Publication Number:
  US 20170349909A1
• Time in Patent Office: 827 Days
• CPC Class Codes: C12N 15/8246 Non-starch polysaccharides, e.g. cellulose, fructans, levans
• CPC Class Codes: C12N 9/1025 Acyltransferases (2.3)

Abstract

The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Claims

1. A method of engineering a plant to decrease the ferulic acid content in the plant, the method comprising:

introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT15 acyltransferase, and wherein the acyltransferase has at least 95% amino acid sequence identity to SEQ ID NO;

4,

culturing the plant under conditions in which the acyltransferase is expressed; and

selecting a plant that has a decreased ferulic acid content compared to a wildtype plant.

2. The method of claim 1, wherein the polynucleotide is operably linked to a promoter endogenous to the plant.

3. The method of claim 1, wherein the expression cassette comprises a promoter to which the polynucleotide is operably linked.

4. The method of claim 3, wherein the promoter is a tissue-specific promoter.

5. The method of claim 4, wherein the promoter drives expression in cell wall.

6. The method of claim 5, wherein the polynucleotide has at least 70% identity to SEQ ID NO:

3.

7. A plant engineered by the method of claim 1, or a progeny of the plant.

9. Biomass comprising the plant or a part of the plant of claim 7.

10. A method of obtaining an increased amount of soluble sugars in a saccharification reaction, the method comprising:

subjecting the plant of claim 7 to a saccharification reaction, thereby increasing the amount of soluble sugars that can be obtained from the plant as compared to a wild-type plant.

11. The method of claim 1, wherein the plant is a grass plant.

12. The method of claim 1, wherein the plant is rice, corn, switchgrass, sorghum, millet, miscanthus, sugarcane, alfalfa, wheat, soy, rye, barley, turfgrass, hemp, bamboo, rape, sunflower, or brachypodium.

13. the method of claim 1, wherein the AT15 acyltransferase comprises SEQ ID NO:

4.

14. The plant of claim 7, wherein the plant is a grass plant.

15. The plant of claim 7, wherein the plant is rice, corn, switchgrass, sorghum, millet, miscanthus, sugarcane, alfalfa, wheat, soy, rye, barley, turfgrass, hemp, bamboo, rape, sunflower, or brachypodium.

16. The plant of claim 7, wherein the AT15 acyltransferase comprises SEQ ID NO:

4.

8. A plant cell comprising a polynucleotide encoding an AT15 acytransferase having at least 95% amino acid sequence identity to SEQ ID NO:

4, operably linked to a heterologous promoter;

or a plant comprising a heterologous polynucleotide encoding an AT15 acyltransferase having at least 95% amino acid sequence identity to SEQ ID NO;

4.