Delivery methods and compositions for nuclease-mediated genome engineering
First Claim
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1. A method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising:
- administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the AAV vector has an AAV6 capsid.
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Abstract
The present disclosure is in the field of genome engineering, particularly targeted modification of the genome of a cell.
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13 Claims
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1. A method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising:
administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the AAV vector has an AAV6 capsid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method of introducing a donor nucleic acid molecule into an isolated cell, the method comprising:
administering to the cell at least one adeno-associated virus (AAV) vector comprising a donor nucleic acid molecule in the presence of at least one inhibitor of Platelet-derived growth factor receptor under conditions sufficient to increase efficiency of viral delivery to the cell, wherein the inhibitor is selected from the group consisting of CP-673451 and Crenolanib and wherein the AAV vector has an AAV6 capsid. - View Dependent Claims (13)
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