Substance measuring method and measuring apparatus using electrochemical biosensor
First Claim
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1. A measuring method using a biosensor, the method comprising:
- introducing a sample including a substance to be measured into an electrochemical measurement cell,wherein the electrochemical measurement cell comprises;
an insulating base plate; and
an electrode system formed on the insulating base plate and comprising two or more electrodes, wherein at least one of the electrodes includes an oxidoreductase;
applying a first voltage to the electrode system;
applying a second voltage to the electrode system;
obtaining a first signal dependent on the amount of the oxidoreductase which is capable of catalyzing an oxidation reduction reaction and contributing to an electron transfer to and from the electrode when the first voltage is applied;
obtaining a second signal dependent on the amount of the substance to be measured in the sample when the second voltage is applied; and
correcting the value of the second signal based on the value of the first signal, whereby to determine the concentration of the substance in the sample,wherein the first voltage is a voltage of not more than the reduction potential of the oxidoreductase, and the second voltage is a voltage of not less than the oxidation potential of the oxidoreductase.
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Abstract
Provided is a measuring method using a biosensor, the method including: introducing a sample containing a substance to be measured into an electrochemical measurement cell, wherein the electrochemical measurement cell comprises: an insulating base plate; and an electrode system applying a first voltage to the electrode system; applying a second voltage to the electrode system; obtaining a first signal; obtaining a second signal; and correcting the second signal by the first signal, to determine the concentration of the substance to be measured in the sample.
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Citations
17 Claims
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1. A measuring method using a biosensor, the method comprising:
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introducing a sample including a substance to be measured into an electrochemical measurement cell, wherein the electrochemical measurement cell comprises; an insulating base plate; and an electrode system formed on the insulating base plate and comprising two or more electrodes, wherein at least one of the electrodes includes an oxidoreductase; applying a first voltage to the electrode system; applying a second voltage to the electrode system; obtaining a first signal dependent on the amount of the oxidoreductase which is capable of catalyzing an oxidation reduction reaction and contributing to an electron transfer to and from the electrode when the first voltage is applied; obtaining a second signal dependent on the amount of the substance to be measured in the sample when the second voltage is applied; and correcting the value of the second signal based on the value of the first signal, whereby to determine the concentration of the substance in the sample, wherein the first voltage is a voltage of not more than the reduction potential of the oxidoreductase, and the second voltage is a voltage of not less than the oxidation potential of the oxidoreductase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A measuring apparatus comprising:
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a biosensor comprising; an electrochemical measurement cell comprising; an insulating base plate; and an electrode system formed on the insulating base plate, and comprising two or more electrodes, wherein at least one of the electrodes includes an oxidoreductase; a control section configured to control the application of a first voltage and a second voltage to the biosensor so that the first voltage is a voltage of not more than the reduction potential of the oxidoreductase, and the second voltage is a voltage of not less than the oxidation potential of the oxidoreductase; a detection section configured to detect a first signal and a second signal obtained by the application of the first voltage and the second voltage to the biosensor; an arithmetic section configured to correct the value of the second signal based on the value of the first signal, to determine the concentration of a substance in a sample; and an output section configured to output the calculated concentration of the substance. - View Dependent Claims (14, 15, 16, 17)
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Specification