×

Substance measuring method and measuring apparatus using electrochemical biosensor

  • US 10,451,577 B2
  • Filed: 03/14/2017
  • Issued: 10/22/2019
  • Est. Priority Date: 03/16/2016
  • Status: Active Grant
First Claim
Patent Images

1. A measuring method using a biosensor, the method comprising:

  • introducing a sample including a substance to be measured into an electrochemical measurement cell,wherein the electrochemical measurement cell comprises;

    an insulating base plate; and

    an electrode system formed on the insulating base plate and comprising two or more electrodes, wherein at least one of the electrodes includes an oxidoreductase;

    applying a first voltage to the electrode system;

    applying a second voltage to the electrode system;

    obtaining a first signal dependent on the amount of the oxidoreductase which is capable of catalyzing an oxidation reduction reaction and contributing to an electron transfer to and from the electrode when the first voltage is applied;

    obtaining a second signal dependent on the amount of the substance to be measured in the sample when the second voltage is applied; and

    correcting the value of the second signal based on the value of the first signal, whereby to determine the concentration of the substance in the sample,wherein the first voltage is a voltage of not more than the reduction potential of the oxidoreductase, and the second voltage is a voltage of not less than the oxidation potential of the oxidoreductase.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×