Reagent kit used for detecting lipoprotein-associated phospholipase A2, and preparation method and application for reagent kit
First Claim
1. A kit for detecting lipoprotein-associated phospholipase A2, comprising one or more first anti-lipoprotein-associated phospholipase A2 antibodies coated on a magnetic sphere for binding with lipoprotein-associated phospholipase A2 to be detected, and one or more second anti-lipoprotein-associated phospholipase A2 antibodies labeled with a trace marker for binding with the lipoprotein-associated phospholipase A2 to be detected at other binding sites different from the binding sites of the first anti-lipoprotein-associated phospholipase A2 antibodies with the lipoprotein-associated phospholipase A2 to be detected;
- wherein the trace marker is at least one selected from the group consisting of luminol and derivatives thereof, isoluminol and derivatives thereof, and acridinium esters; and
wherein the kit further comprises a displacer including water as a solvent and the following components;
at least one selected from the group consisting of 3-[3-(cholamidopropyl) dimethylammonio] propanesulfonate, 8-anilino-1-naphthalenesulfonic acid, sodium deoxycholate, barbiturate, and acetic acid;
newborn bovine serum;
goat serum and/or horse serum;
dithiothreitol;
tris (hydroxymethyl) aminomethane;
hydrated 2-morpholinoethanesulfonic acid;
casein; and
disodium ethylenediaminetetraacetate.
1 Assignment
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Accused Products
Abstract
A reagent kit used for detecting lipoprotein-associated phospholipase A2, and a preparation method for the reagent kit. The reagent kit comprises one or a plurality of first anti-lipoprotein-associated phospholipase A2 antibodies used for binding lipoprotein-associated phospholipase A2 to be measured, and one or a plurality of second anti-lipoprotein-associated phospholipase A2 antibodies marked with a trace marker and binding with the lipoprotein-associated phospholipase A2 to be measured at another site, different from the binding site of the lipoprotein-associated phospholipase A2 to be measured and the first anti-lipoprotein-associated phospholipase A2 antibodies. The reagent kit also comprises a displacing agent, so as to further increase the detection accuracy of the reagent kit. A method using the reagent kit for the detection of lipoprotein-associated phospholipase A2 may take serum as a detection sample, has high repeatability and high accuracy, and measures the concentration of lipoprotein-associated phospholipase A2 in the sample in a highly sensitive manner.
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Citations
20 Claims
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1. A kit for detecting lipoprotein-associated phospholipase A2, comprising one or more first anti-lipoprotein-associated phospholipase A2 antibodies coated on a magnetic sphere for binding with lipoprotein-associated phospholipase A2 to be detected, and one or more second anti-lipoprotein-associated phospholipase A2 antibodies labeled with a trace marker for binding with the lipoprotein-associated phospholipase A2 to be detected at other binding sites different from the binding sites of the first anti-lipoprotein-associated phospholipase A2 antibodies with the lipoprotein-associated phospholipase A2 to be detected;
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wherein the trace marker is at least one selected from the group consisting of luminol and derivatives thereof, isoluminol and derivatives thereof, and acridinium esters; and wherein the kit further comprises a displacer including water as a solvent and the following components; at least one selected from the group consisting of 3-[3-(cholamidopropyl) dimethylammonio] propanesulfonate, 8-anilino-1-naphthalenesulfonic acid, sodium deoxycholate, barbiturate, and acetic acid; newborn bovine serum; goat serum and/or horse serum; dithiothreitol; tris (hydroxymethyl) aminomethane; hydrated 2-morpholinoethanesulfonic acid; casein; and disodium ethylenediaminetetraacetate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for preparing a kit for detecting lipoprotein-associated phospholipase A2, comprising:
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directly or indirectly coating a first anti-lipoprotein-associated phospholipase A2 antibody on magnetic sphere, and directly or indirectly labeling a second anti-lipoprotein-associated phospholipase A2 antibody with a trace marker, to obtain the kit including one or more first anti-lipoprotein-associated phospholipase A2 antibodies coated on magnetic sphere for binding with lipoprotein-associated phospholipase A2 to be detected, and one or more second anti-lipoprotein-associated phospholipase A2 antibodies labeled with a trace marker for binding with lipoprotein-associated phospholipase A2 to be detected at other binding sites different from the binding sites of the first anti-Lipoprotein-associated phospholipase A2 antibodies with lipoprotein-associated phospholipase A2 to be detected; wherein the trace marker is at least one selected from the group consisting of luminol and its derivatives, isoluminol and its derivatives, and acridinium esters; and wherein the method further comprises a step of preparing a displacer as a component of the kit, including;
mixing water as a solvent and the following components;at least one selected from the group consisting of 3-[3-(cholamidopropyl) dimethylammonio] propanesulfonate, 8-anilino-1-naphthalenesulfonic acid, sodium deoxycholate, barbiturate, and acetic acid; newborn bovine serum; goat serum and/or horse serum; dithiothreitol; tris (hydroxymethyl) aminomethane; hydrated 2-morpholinoethanesulfonic acid; casein; and disodium ethylenediaminetetraacetate. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method for detecting lipoprotein-associated phospholipase A2, wherein the method comprises using a kit for detecting lipoprotein-associated phospholipase A2 to detect a concentration of lipoprotein-associated phospholipase A2 in a subject sample by chemiluminescence immunoassay, wherein the kit includes one or more first anti-lipoprotein-associated phospholipase A2 antibodies coated on magnetic sphere for binding with lipoprotein-associated phospholipase A2 to be detected, and one or more second anti-lipoprotein-associated phospholipase A2 antibodies labeled with a trace marker for binding with lipoprotein-associated phospholipase A2 to be detected at other binding sites different from the binding sites of the first anti-Lipoprotein-associated phospholipase A2 antibodies with lipoprotein-associated phospholipase A2 to be detected;
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wherein the kit further comprises a displacer including water as a solvent and the following components; at least one selected from the group consisting of 3-[3-(cholamidopropyl) dimethylammonio] propanesulfonate, 8-anilino-1-naphthalenesulfonic acid, sodium deoxycholate, barbiturate, and acetic acid; newborn bovine serum; goat serum and/or horse serum; dithiothreitol; tris (hydroxymethyl) aminomethane; hydrated 2-morpholinoethanesulfonic acid; casein; and disodium ethylenediaminetetraacetate. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification