Compositions for biological systems and methods for preparing and using the same

• US 10,456,423 B2
• Filed: 05/24/2018
• Issued: 10/29/2019
• Est. Priority Date: 06/13/2016
• Status: Active Grant

• Current Assignee: Smart Surgical, Inc.
• Original Assignee: Smart Surgical, Inc.
• Inventors: Jones, Christopher D., Banerjee Mustafi, Soumyajit
• Primary Examiner(s): Hobbs, Lisa J
• Application Number:
  US15/988,752
• Publication Number:
  US 20180264044A1
• Time in Patent Office: 523 Days
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 38/1808 Epidermal growth factor [EGF] urogastrone
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: A61K 38/185 Nerve growth factor [NGF]; Brain derived neurotrophic factor [BDNF]; Ciliary neurotrophic factor [CNTF]; Glial derived neurotrophic factor [GDNF]; Neurotrophins, e.g. NT-3
• CPC Class Codes: A61K 38/1866 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: A61K 38/191 Tumor necrosis factors [TNF], e.g. lymphotoxin [LT], i.e. TNF-beta
• CPC Class Codes: A61K 38/195 Chemokines, e.g. RANTES
• CPC Class Codes: A61K 38/204 IL-6
• CPC Class Codes: A61K 38/2046 IL-7
• CPC Class Codes: A61K 38/206 IL-9
• CPC Class Codes: A61K 38/2086 IL-13 to IL-16
• CPC Class Codes: A61K 38/212 IFN-alpha
• CPC Class Codes: A61K 38/217 IFN-gamma
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 47/42 Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein oligopeptides having up to five amino acids A61K47/183; polyamino acids A61K47/34
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L

Abstract

A composition for influencing biological growth including live cells, a fluid comprising dextrose, a protectant, and a first cytokine having a first concentration within the composition as described within the present disclosure.

Claims

1. A composition for influencing biological growth comprising:

a low pH fluid base, wherein the low pH fluid base comprises dextrose;

mononuclear cells obtained from human umbilical cord blood;

VEGF-A, at a concentration within the composition of between 481 picograms per ml and 895 picograms per ml;

PDGF-BB, at a concentration within the composition of between 807 picograms per ml and 1500 picograms per ml;

EGF, at a concentration within the composition of between 715 picograms per ml and 1329 picograms per ml;

SCF, at a concentration within the composition of between 23 picograms per ml and 45 picograms per ml; and

IL-1RA, at a concentration within the composition of between 2215 picograms per ml and 4114 picograms per ml,

wherein the composition is configured for implantation within a human subject.

2. The composition of claim 1, wherein:

the concentration within the composition of VEGF-A is between 516 picograms per ml and 861 picograms per ml;

the concentration within the composition of PDGF-BB is between 865 picograms per ml and 1442 picograms per ml;

the concentration within the composition of EGF is between 766 picograms per ml and 1278 picograms per ml;

the concentration within the composition of SCF is between 25 picograms per ml and 43 picograms per ml; and

the concentration within the composition of IL-1RA is between 2373 picograms per ml and 3956 picograms per ml.

3. The composition of claim 1, wherein:

the concentration within the composition of VEGF-A is between 550 picograms per ml and 826 picograms per ml;

the concentration within the composition of PDGF-BB is between 922 picograms per ml and 1385 picograms per ml;

the concentration within the composition of EGF is between 817 picograms per ml and 1227 picograms per ml;

the concentration within the composition of SCF is between 27 picograms per ml and 42 picograms per ml; and

the concentration within the composition of IL-1RA is between 2531 picograms per ml and 3798 picograms per ml.

4. The composition of claim 1, wherein:

the concentration within the composition of VEGF-A is between 585 picograms per ml and 792 picograms per ml;

the concentration within the composition of PDGF-BB is between 980 picograms per ml and 1327 picograms per ml;

the concentration within the composition of EGF is between 868 picograms per ml and 1176 picograms per ml;

the concentration within the composition of SCF is between 29 picograms per ml and 40 picograms per ml; and

the concentration within the composition of IL-1RA is between 2689 picograms per ml and 3640 picograms per ml.

5. The composition of claim 1, wherein:

the concentration within the composition of VEGF-A is between 619 picograms per ml and 758 picograms per ml;

the concentration within the composition of PDGF-BB is between 1038 picograms per ml and 1269 picograms per ml;

the concentration within the composition of EGF is between 919 picograms per ml and 1124 picograms per ml;

the concentration within the composition of SCF is between 30 picograms per ml and 38 picograms per ml; and

the concentration within the composition of IL-1RA is between 2848 picograms per ml and 3481 picograms per ml.

6. The composition of claim 1, wherein:

the concentration within the composition of VEGF-A is between 653 picograms per ml and 723 picograms per ml;

the concentration within the composition of PDGF-BB is between 1095 picograms per ml and 1212 picograms per ml;

the concentration within the composition of EGF is between 970 picograms per ml and 1073 picograms per ml;

the concentration within the composition of SCF is between 32 picograms per ml and 36 picograms per ml; and

the concentration within the composition of IL-1RA is between 3006 picograms per ml and 3323 picograms per ml, and wherein the composition further comprises;

GM-CSF having a concentration within the composition of between 10 picograms per ml and 12 picograms per ml;

IL-4 having a concentration within the composition of between 23 picograms per ml and 27 picograms per ml;

IL-8 having a concentration within the composition of between 6113 picograms per ml and 6757 picograms per ml;

SDF-1α

having a concentration within the composition of between 1512 picograms per ml and 1672 picograms per ml; and

RANTES having a concentration within the composition of between 4447 picograms per ml and 4916 picograms per ml.

13. The composition of claim 1, wherein the low pH fluid base further comprises dextran.

14. The composition of claim 1, further comprising albumin.

7. A composition for influencing biological growth comprising:

a low pH fluid base, wherein the low pH fluid base comprises dextrose;

mononuclear cells obtained from human umbilical cord blood;

and

wherein the composition includes the following cytokines with their concentration within the composition;

BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GM-CSF at between 7 picograms per ml and 15 picograms per ml, Gro-α

at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α

at between 253 picograms per ml and 472 picograms per ml, IFN-γ

at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-1α

at between 712 picograms per ml and 1324 picograms per ml, IL-1β

at between 14 picograms per ml and 28 picograms per ml, IL-1RA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-1α

at between 303 picograms per ml and 565 picograms per ml, MIP-1β

at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-1α

at between 1114 picograms per ml and 2071 picograms per ml, TNF-α

at between 42 picograms per ml and 80 picograms per ml, TNF-β

at between 12 picograms per ml and 25 picograms per ml, VEGF-A at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml,

wherein the composition is configured for implantation within a human subject.

8. The composition of claim 7, wherein the concentrations of the cytokines are:

BDNF at between 527 picograms per ml and 880 picograms per ml, bNGF at between 15 picograms per ml and 25 picograms per ml, EGF at between 766 picograms per ml and 1278 picograms per ml, Eotaxin at between 39 picograms per ml and 67 picograms per ml, FGF-2 at between 30 picograms per ml and 51 picograms per ml, GM-CSF at between 8 picograms per ml and 15 picograms per ml, Gro-α

at between 3186 picograms per ml and 5312 picograms per ml, HGF at between 378 picograms per ml and 631 picograms per ml, IFN-α

at between 271 picograms per ml and 454 picograms per ml, IFN-γ

at between 35 picograms per ml and 60 picograms per ml, IL-10 at between 72 picograms per ml and 121 picograms per ml, IL-12p70 at between 10 picograms per ml and 18 picograms per ml, IL-13 at between 12 picograms per ml and 21 picograms per ml, IL-15 at between 32 picograms per ml and 55 picograms per ml, IL-17A at between 21 picograms per ml and 35 picograms per ml, IL-18 at between 83 picograms per ml and 140 picograms per ml, IL-1α

at between 763 picograms per ml and 1273 picograms per ml, IL-1β

at between 15 picograms per ml and 27 picograms per ml, IL-1RA at between 2373 picograms per ml and 3956 picograms per ml, IL-2 at between 17 picograms per ml and 30 picograms per ml, IL-21 at between 396 picograms per ml and 662 picograms per ml, IL-22 at between 17 picograms per ml and 30 picograms per ml, IL-23 at between 216 picograms per ml and 361 picograms per ml, IL-27 at between 22 picograms per ml and 38 picograms per ml, IL-31 at between 12 picograms per ml and 21 picograms per ml, IL-4 at between 18 picograms per ml and 32 picograms per ml, IL-5 at between 16 picograms per ml and 27 picograms per ml, IL-6 at between 54 picograms per ml and 91 picograms per ml, IL-7 at between 28 picograms per ml and 48 picograms per ml, IL-8 at between 4826 picograms per ml and 8044 picograms per ml, IL-9 at between 141 picograms per ml and 236 picograms per ml, IP-10 at between 503 picograms per ml and 840 picograms per ml, LIF at between 44 picograms per ml and 74 picograms per ml, MCP-1 at between 15,514 picograms per ml and 25,859 picograms per ml, MIP-1α

at between 325 picograms per ml and 543 picograms per ml, MIP-1β

at between 402 picograms per ml and 671 picograms per ml, PDGF-BB at between 865 picograms per ml and 1442 picograms per ml, PIGF-1 at between 796 picograms per ml and 1328 picograms per ml, RANTES at between 3511 picograms per ml and 5853 picograms per ml, SCF at between 25 picograms per ml and 43 picograms per ml, SDF-1α

at between 1194 picograms per ml and 1991 picograms per ml, TNF-α

at between 45 picograms per ml and 77 picograms per ml, TNF-β

at between 13 picograms per ml and 24 picograms per ml, VEGF-A at between 516 picograms per ml and 861 picograms per ml, and VEGF-D at between 50 picograms per ml and 84 picograms per ml.

9. The composition of claim 7, wherein the concentrations of the cytokines are:

BDNF at between 562 picograms per ml and 844 picograms per ml, bNGF at between 16 picograms per ml and 24 picograms per ml, EGF at between 817 picograms per ml and 1227 picograms per ml, Eotaxin at between 42 picograms per ml and 64 picograms per ml, FGF-2 at between 32 picograms per ml and 49 picograms per ml, GM-CSF at between 9 picograms per ml and 14 picograms per ml, Gro-α

at between 3399 picograms per ml and 5099 picograms per ml, HGF at between 403 picograms per ml and 605 picograms per ml, IFN-α

at between 290 picograms per ml and 436 picograms per ml, IFN-γ

at between 38 picograms per ml and 57 picograms per ml, IL-10 at between 77 picograms per ml and 117 picograms per ml, IL-12p70 at between 11 picograms per ml and 18 picograms per ml, IL-13 at between 13 picograms per ml and 20 picograms per ml, IL-15 at between 34 picograms per ml and 53 picograms per ml, IL-17A at between 22 picograms per ml and 34 picograms per ml, IL-18 at between 89 picograms per ml and 135 picograms per ml, IL-1α

at between 814 picograms per ml and 1222 picograms per ml, IL-1β

at between 17 picograms per ml and 26 picograms per ml, IL-1RA at between 2531 picograms per ml and 3798 picograms per ml, IL-2 at between 18 picograms per ml and 29 picograms per ml, IL-21 at between 423 picograms per ml and 636 picograms per ml, IL-22 at between 19 picograms per ml and 29 picograms per ml, IL-23 at between 230 picograms per ml and 347 picograms per ml, IL-27 at between 24 picograms per ml and 37 picograms per ml, IL-31 at between 13 picograms per ml and 20 picograms per ml, IL-4 at between 19 picograms per ml and 30 picograms per ml, IL-5 at between 17 picograms per ml and 26 picograms per ml, IL-6 at between 58 picograms per ml and 88 picograms per ml, IL-7 at between 30 picograms per ml and 46 picograms per ml, IL-8 at between 5147 picograms per ml and 7722 picograms per ml, IL-9 at between 150 picograms per ml and 226 picograms per ml, IP-10 at between 537 picograms per ml and 806 picograms per ml, LIF at between 47 picograms per ml and 71 picograms per ml, MCP-1 at between 16,549 picograms per ml and 24,824 picograms per ml, MIP-1α

at between 347 picograms per ml and 522 picograms per ml, MIP-1β

at between 429 picograms per ml and 644 picograms per ml, PDGF-BB at between 922 picograms per ml and 1385 picograms per ml, PIGF-1 at between 849 picograms per ml and 1275 picograms per ml, RANTES at between 3745 picograms per ml and 5619 picograms per ml, SCF at between 27 picograms per ml and 42 picograms per ml, SDF-1α

at between 1273 picograms per ml and 1911 picograms per ml, TNF-α

at between 49 picograms per ml and 74 picograms per ml, TNF-β

at between 14 picograms per ml and 23 picograms per ml, VEGF-A at between 550 picograms per ml and 826 picograms per ml, and VEGF-D at between 53 picograms per ml and 81 picograms per ml.

10. The composition of claim 7, wherein the concentrations of the cytokines are:

BDNF at between 597 picograms per ml and 809 picograms per ml, bNGF at between 17 picograms per ml and 23 picograms per ml, EGF at between 868 picograms per ml and 1176 picograms per ml, Eotaxin at between 45 picograms per ml and 61 picograms per ml, FGF-2 at between 34 picograms per ml and 47 picograms per ml, GM-CSF at between 9 picograms per ml and 13 picograms per ml, Gro-α

at between 3611 picograms per ml and 4887 picograms per ml, HGF at between 428 picograms per ml and 580 picograms per ml, IFN-α

at between 308 picograms per ml and 418 picograms per ml, IFN-γ

at between 40 picograms per ml and 55 picograms per ml, IL-10 at between 82 picograms per ml and 112 picograms per ml, IL-12p70 at between 12 picograms per ml and 17 picograms per ml, IL-13 at between 13 picograms per ml and 19 picograms per ml, IL-15 at between 36 picograms per ml and 51 picograms per ml, IL-17A at between 23 picograms per ml and 33 picograms per ml, IL-18 at between 95 picograms per ml and 129 picograms per ml, IL-1α

at between 865 picograms per ml and 1171 picograms per ml, IL-1α

at between 18 picograms per ml and 25 picograms per ml, IL-1RA at between 2689 picograms per ml and 3640 picograms per ml, IL-2 at between 20 picograms per ml and 28 picograms per ml, IL-21 at between 449 picograms per ml and 609 picograms per ml, IL-22 at between 20 picograms per ml and 28 picograms per ml, IL-23 at between 245 picograms per ml and 332 picograms per ml, IL-27 at between 25 picograms per ml and 35 picograms per ml, IL-31 at between 13 picograms per ml and 19 picograms per ml, IL-4 at between 21 picograms per ml and 29 picograms per ml, IL-5 at between 18 picograms per ml and 25 picograms per ml, IL-6 at between 61 picograms per ml and 84 picograms per ml, IL-7 at between 32 picograms per ml and 44 picograms per ml, IL-8 at between 5469 picograms per ml and 7401 picograms per ml, IL-9 at between 160 picograms per ml and 217 picograms per ml, IP-10 at between 570 picograms per ml and 772 picograms per ml, LIF at between 50 picograms per ml and 68 picograms per ml, MCP-1 at between 17,583 picograms per ml and 23,790 picograms per ml, MIP-1α

at between 369 picograms per ml and 500 picograms per ml, MIP-1β

at between 455 picograms per ml and 617 picograms per ml, PDGF-BB at between 980 picograms per ml and 1327 picograms per ml, PIGF-1 at between 902 picograms per ml and 1222 picograms per ml, RANTES at between 3979 picograms per ml and 5385 picograms per ml, SCF at between 29 picograms per ml and 40 picograms per ml, SDF-1α

at between 1353 picograms per ml and 1832 picograms per ml, TNF-α

at between 52 picograms per ml and 71 picograms per ml, TNF-β

at between 15 picograms per ml and 22 picograms per ml, VEGF-A at between 585 picograms per ml and 792 picograms per ml, and VEGF-D at between 56 picograms per ml and 78 picograms per ml.

11. The composition of claim 7, wherein the concentrations of the cytokines are:

BDNF at between 632 picograms per ml and 774 picograms per ml, bNGF at between 18 picograms per ml and 22 picograms per ml, EGF at between 919 picograms per ml and 1124 picograms per ml, Eotaxin at between 47 picograms per ml and 59 picograms per ml, FGF-2 at between 36 picograms per ml and 45 picograms per ml, GM-CSF at between 10 picograms per ml and 13 picograms per ml, Gro-α

at between 3823 picograms per ml and 4674 picograms per ml, HGF at between 453 picograms per ml and 555 picograms per ml, IFN-α

at between 326 picograms per ml and 399 picograms per ml, IFN-γ

at between 42 picograms per ml and 53 picograms per ml, IL-10 at between 87 picograms per ml and 107 picograms per ml, IL-12p70 at between 12 picograms per ml and 16 picograms per ml, IL-13 at between 14 picograms per ml and 19 picograms per ml, IL-15 at between 39 picograms per ml and 48 picograms per ml, IL-17A at between 25 picograms per ml and 31 picograms per ml, IL-18 at between 100 picograms per ml and 124 picograms per ml, IL-1α

at between 916 picograms per ml and 1121 picograms per ml, IL-1β

at between 19 picograms per ml and 24 picograms per ml, IL-1RA at between 2848 picograms per ml and 3481 picograms per ml, IL-2 at between 21 picograms per ml and 26 picograms per ml, IL-21 at between 476 picograms per ml and 583 picograms per ml, IL-22 at between 21 picograms per ml and 27 picograms per ml, IL-23 at between 259 picograms per ml and 318 picograms per ml, IL-27 at between 27 picograms per ml and 34 picograms per ml, IL-31 at between 14 picograms per ml and 18 picograms per ml, IL-4 at between 22 picograms per ml and 28 picograms per ml, IL-5 at between 19 picograms per ml and 24 picograms per ml, IL-6 at between 65 picograms per ml and 81 picograms per ml, IL-7 at between 34 picograms per ml and 42 picograms per ml, IL-8 at between 5791 picograms per ml and 7079 picograms per ml, IL-9 at between 169 picograms per ml and 208 picograms per ml, IP-10 at between 604 picograms per ml and 739 picograms per ml, LIF at between 53 picograms per ml and 66 picograms per ml, MCP-1 at between 18,617 picograms per ml and 22,756 picograms per ml, MIP-1α

at between 390 picograms per ml and 478 picograms per ml, MIP-1β

at between 482 picograms per ml and 590 picograms per ml, PDGF-BB at between 1038 picograms per ml and 1269 picograms per ml, PIGF-1 at between 955 picograms per ml and 1169 picograms per ml, RANTES at between 4213 picograms per ml and 5151 picograms per ml, SCF at between 30 picograms per ml and 38 picograms per ml, SDF-1α

at between 1433 picograms per ml and 1752 picograms per ml, TNF-α

at between 55 picograms per ml and 68 picograms per ml, TNF-β

at between 16 picograms per ml and 21 picograms per ml, VEGF-A at between 619 picograms per ml and 758 picograms per ml, and VEGF-D at between 60 picograms per ml and 74 picograms per ml.

12. The composition of claim 7, wherein the concentrations of the cytokines are:

BDNF at between 668 picograms per ml and 739 picograms per ml, bNGF at between 19 picograms per ml and 21 picograms per ml, EGF at between 970 picograms per ml and 1073 picograms per ml, Eotaxin at between 50 picograms per ml and 56 picograms per ml, FGF-2 at between 38 picograms per ml and 43 picograms per ml, GM-CSF at between 10 picograms per ml and 12 picograms per ml, Gro-α

at between 4036 picograms per ml and 4462 picograms per ml, HGF at between 478 picograms per ml and 530 picograms per ml, IFN-α

at between 344 picograms per ml and 381 picograms per ml, IFN-γ

at between 45 picograms per ml and 50 picograms per ml, IL-10 at between 91 picograms per ml and 102 picograms per ml, IL-12p70 at between 13 picograms per ml and 15 picograms per ml, IL-13 at between 15 picograms per ml and 18 picograms per ml, IL-15 at between 41 picograms per ml and 46 picograms per ml, IL-17A at between 26 picograms per ml and 30 picograms per ml, IL-18 at between 106 picograms per ml and 118 picograms per ml, IL-1α

at between 967 picograms per ml and 1070 picograms per ml, IL-1β

at between 20 picograms per ml and 23 picograms per ml, IL-1RA at between 3006 picograms per ml and 3323 picograms per ml, IL-2 at between 22 picograms per ml and 25 picograms per ml, IL-21 at between 502 picograms per ml and 556 picograms per ml, IL-22 at between 22 picograms per ml and 26 picograms per ml, IL-23 at between 274 picograms per ml and 303 picograms per ml, IL-27 at between 28 picograms per ml and 32 picograms per ml, IL-31 at between 15 picograms per ml and 18 picograms per ml, IL-4 at between 23 picograms per ml and 27 picograms per ml, IL-5 at between 20 picograms per ml and 23 picograms per ml, IL-6 at between 69 picograms per ml and 77 picograms per ml, IL-7 at between 36 picograms per ml and 40 picograms per ml, IL-8 at between 6113 picograms per ml and 6757 picograms per ml, IL-9 at between 178 picograms per ml and 198 picograms per ml, IP-10 at between 637 picograms per ml and 705 picograms per ml, LIF at between 56 picograms per ml and 63 picograms per ml, MCP-1 at between 19,652 picograms per ml and 21,721 picograms per ml, MIP-1α

at between 412 picograms per ml and 456 picograms per ml, MIP-1β

at between 509 picograms per ml and 564 picograms per ml, PDGF-BB at between 1095 picograms per ml and 1212 picograms per ml, PIGF-1 at between 1009 picograms per ml and 1116 picograms per ml, RANTES at between 4447 picograms per ml and 4916 picograms per ml, SCF at between 32 picograms per ml and 36 picograms per ml, SDF-1α

at between 1512 picograms per ml and 1672 picograms per ml, TNF-α

at between 58 picograms per ml and 65 picograms per ml, TNF-β

at between 17 picograms per ml and 20 picograms per ml, VEGF-A at between 653 picograms per ml and 723 picograms per ml, and VEGF-D at between 63 picograms per ml and 71 picograms per ml.

15. The composition of claim 7, wherein the low pH fluid base further comprises dextran.

16. The composition of claim 7, further comprising albumin.