Pancreatic endocrine cells and methods thereof
First Claim
1. A method for generating human pancreatic endocrine cells, comprising differentiating pancreatic endoderm cells into pancreatic endocrine cells by culturing the pancreatic endoderm cells with a medium supplemented with Exendin-4, then removing the medium supplemented with Exendin-4 and subsequently culturing the cells in medium supplemented with hepatocyte growth factor (HGF), Exendin-1 and insulin growth factor (IGF)-1.
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Abstract
The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
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Citations
15 Claims
- 1. A method for generating human pancreatic endocrine cells, comprising differentiating pancreatic endoderm cells into pancreatic endocrine cells by culturing the pancreatic endoderm cells with a medium supplemented with Exendin-4, then removing the medium supplemented with Exendin-4 and subsequently culturing the cells in medium supplemented with hepatocyte growth factor (HGF), Exendin-1 and insulin growth factor (IGF)-1.
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8. A method for differentiating pluripotent stem cells into pancreatic endocrine cells, comprising the steps of:
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a. Differentiating human pluripotent stem cells into definitive endoderm cells by treating the pluripotent cells with activin A; b. Differentiating the definitive endoderm cells into pancreatic endoderm cells by treating the definitive endoderm cells with at least one fibroblast growth factor, or with retinoic acid and at least one fibroblast growth factor (FGF); and c. Differentiating the pancreatic endoderm cells into pancreatic endocrine cells by treating the pancreatic endoderm cells with Exendin-4, then removing the medium supplemented with Exendin-4 and subsequently culturing the cells in medium supplemented with hepatocyte growth factor (HGF), Exendin-1 and insulin growth factor (IGF)-1. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15)
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Specification