Apparatus for disruption of cell and tissue samples in multi-well plates

• US 10,456,788 B2
• Filed: 01/28/2019
• Issued: 10/29/2019
• Est. Priority Date: 01/26/2018
• Status: Active Grant

• Current Assignee: Ilya Alexandrov, Michael C. Sherman, Yehuda Buff, Yury Sherman
• Original Assignee: Ilya Alexandrov, Michael C. Sherman, Yehuda Buff, Yury Sherman
• Inventors: Sherman, Yury, Sherman, Michael, Alexandrov, Ilya, Buff, Yehuda
• Primary Examiner(s): White, Dennis
• Application Number:
  US16/259,766
• Publication Number:
  US 20190232295A1
• Time in Patent Office: 274 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/04 Closures and closing means
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C

Abstract

The present invention relates to an apparatus for substantially horizontally oscillating one or more multi-well plates containing a liquid, a solid, or a mixture thereof that operates to thoroughly disrupt solid substances in multi-well plates. The apparatus provides oscillation of plates in a horizontal direction through the use of springs and rotating mechanical components. The apparatus includes a circular rotating ring having a toothed circumference that rotates horizontally, plate holder, and a spring attached to the housing and in constant contact with the plate holder. The apparatus can be used in a cold room or placed in a refrigerator to keep the substances at low temperature and to eliminate noise when the disruptor operates.

Claims

1. An apparatus for disrupting substances contained in multi-well plates, said apparatus comprising:

a housing;

one or more guide rod attached to said housing;

one or more box holder attached to one or more guide rod slider, wherein each of said one or more guide rod slider are movably attached to the one or more guide rod;

one or more compression spring each located on one of said one or more guide rod, wherein each of said one or more compression spring on the guide rod is located in-between said housing and said box holder;

an electric motor;

a ring having one or more circumferential tooth, wherein said ring is configured to be spun by said electric motor; and

at least one wedge located on each of said one or more box holder, wherein said wedge and said one or more box holders configured to being continuously spring biased by said one or more compression springs into engagement with the one or more circumferential tooth.

2. The apparatus of claim 1, further comprising a cooperable means on said one or more box holder and multi-well plates configures to releasably attach to said one or more box holder.

3. The apparatus of claim 1, wherein said one or more box holder further comprises an attachable multi-well plate.

4. The apparatus of claim 3, wherein the multi-well plate further comprises a first cover, a second cover and an elastic gasket.

5. The apparatus of claim 1, wherein said housing further comprises spread apart walled portions that are substantially parallel to one another.

6. The apparatus of claim 1, wherein the ring has four circumferential teeth and in-between said teeth is a step.

7. The apparatus of claim 1, wherein said one or more box holder and an attachable multi-well plate have cooperable tongue and groove formations to enable the multi-well plate to be slidably inserted onto the one or more box holder inside the housing, and to be captive therein for effecting simultaneous reciprocation of the multi-well plate with reciprocation of the box holder.

8. The apparatus of claim 1, wherein said one or more guide rod further comprises a guide rod stop.

9. The apparatus of claim 1, wherein said housing further comprises a central holder, wherein said central holder houses said electric motor.